El-Brashy A, Eid M, Talaat W
Department of Analytical Chemistry, Faculty of Pharmacy, Mansorua University, Mansoura 35516, Egypt.
Int J Biomed Sci. 2006 Dec;2(4):406-13.
A simple and sensitive kinetic method is described for the determination of ketoprofen in pure form, pharmaceuticals and biological fluids. The method utilizes an oxidative- coupling reaction based upon oxidation of 3-methyl-2-benzo-thiazolinone hydrazone hydrochloride (MBTH) with Ce(IV) in presence of HCl, where an electrophilic intermediate (diazonium salt of the reagent) is produced, then couples with ketoprofen yielding a highly colored condensation product. The absorbance is measured after 20 min at 605 nm. Calibration graph was linear over the concentration range of 1-8μg/mL with a minimum detection limit of 0.07 μg/mL. The proposed method was applied successfully to the determination of Ketoprofen in pharmaceutical preparations, plasma and urine. The % recoveries were 100.11 for pure form, 100.10 for tablets and gel, 100.0 for suspension and suppositories, 100.2 for capsules and ampoules and 99.79, 99.9 for plasma and urine. The results obtained were in good agreement with those obtained using reference methods for comparison.
描述了一种简单灵敏的动力学方法，用于测定纯品、药物制剂和生物流体中的酮洛芬。该方法利用基于盐酸3 - 甲基 - 2 - 苯并噻唑啉酮腙（MBTH）在HCl存在下被Ce(IV)氧化的氧化偶联反应，在此过程中产生亲电中间体（试剂的重氮盐），然后与酮洛芬偶联生成高显色缩合产物。在605 nm处20分钟后测量吸光度。校准曲线在1 - 8μg/mL浓度范围内呈线性，最低检测限为0.07μg/mL。所提出的方法成功应用于药物制剂、血浆和尿液中酮洛芬的测定。纯品的回收率为100.11%，片剂和凝胶剂为100.10%，混悬剂和栓剂为100.0%，胶囊剂和安瓿剂为100.2%，血浆和尿液分别为99.79%、99.9%。所得结果与使用参考方法获得的结果非常一致。
