Vaezjalali Maryam, Rashidpour Shabnam, Rezaee Hanieh, Hajibeigi Bashir, Zeidi Majid, Gachkar Latif, Aghamohamad Shadi, Najafi Ronak, Goudarzi Hossein
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran ; Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
Hepat Mon. 2013 Mar 17;13(3):e6590. doi: 10.5812/hepatmon.6590. Print 2013 Mar.
Presence of occult hepatitis B infection (OBI) renders HBs antigen (HBsAg) undetectable by ELISA. Therefore it is valuable to evaluate the frequency of OBI among healthy blood donors to improve and perhaps change the strategies of blood screening to reduce the risk of HBV transmission.
The aim of this study was to determine the presence of HBcAb and HBV DNA among Iranian HBsAg negative healthy blood donors who donated their blood to the Tehran Blood Transfusion Center during 2011.
1000 serum specimens negative for HBsAg, HCV antibody and HIV antibody were collected from healthy blood donors and tested for HBcAb. Presence of hepatitis B viral DNA was checked in HBcAb positive samples by nested PCR with two sets of primers to amplify part of HBV S gene.
There were 64 women and 936 men in the population under study. The mean ± SD age of the donors was 38 ± 11 years. 80 out of 1000 samples (8%) were found to be positive for HBcAb. HBV DNA was detected in 50% of HBcAb positive specimens. The mean ± SD age of donors without HBV DNA was 37.7 ± 10.5 years and for donors with HBV DNA was 40.9 ± 11.2 years (P = 0.05).
OBI was prevalent among 50% of HBcAb positive healthy blood donors. The frequency of positive HBcAb among healthy HBsAg negative blood donors was comparable to previous studies reported from Iran. On the other hand, the frequency of HBV DNA in HBsAg negative blood donors was higher than previous reports.
隐匿性乙型肝炎感染（OBI）会导致酶联免疫吸附测定（ELISA）检测不到乙肝表面抗原（HBsAg）。因此，评估健康献血者中OBI的发生率对于改进甚至改变血液筛查策略以降低乙肝病毒（HBV）传播风险具有重要价值。
本研究旨在确定2011年期间向德黑兰输血中心献血的伊朗HBsAg阴性健康献血者中乙肝核心抗体（HBcAb）和HBV DNA的存在情况。
从健康献血者中收集1000份HBsAg、丙型肝炎病毒（HCV）抗体和人类免疫缺陷病毒（HIV）抗体均为阴性的血清标本，并检测HBcAb。通过巢式聚合酶链反应（PCR），使用两组引物扩增HBV S基因的部分片段，对HBcAb阳性样本进行乙肝病毒DNA检测。
研究人群中有64名女性和936名男性。献血者的平均年龄±标准差为38±11岁。1000份样本中有80份（8%）HBcAb呈阳性。50%的HBcAb阳性标本检测到HBV DNA。无HBV DNA的献血者平均年龄±标准差为37.7±10.5岁，有HBV DNA的献血者平均年龄为40.9±11.2岁（P = 0.05）。
50%的HBcAb阳性健康献血者中存在OBI。健康HBsAg阴性献血者中HBcAb阳性的发生率与伊朗此前报道的研究结果相当。另一方面，HBsAg阴性献血者中HBV DNA的发生率高于此前报道。
