Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Mol Microbiol. 2013 Jul;89(1):113-22. doi: 10.1111/mmi.12262. Epub 2013 Jun 5.
A growing class of proteins regulates transcription through interaction with DNA-dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase-binding protein that influences the expression of genes under the control of the late-acting, sporulation sigma factor σ(G) in Bacillus subtilis. Spores from a ylyA mutant exhibited defects in germination corresponding to changes in the levels of membrane receptors for spore germinants and a protein channel governing the release of dipicolinic acid and hydration of the spore core during germination. Purified YlyA interacted with RNA polymerase and stimulated transcription from promoters dependent on σ(G) but not promoters dependent on the housekeeping sigma factor σ(A) . YlyA is a previously unrecognized RNA polymerase-binding protein that is dedicated to modulating the expression of genes involved in spore germination.
越来越多的蛋白质通过与 DNA 依赖性 RNA 聚合酶相互作用来调节转录。在这里，我们报告说，最近发现的高度保守的孢子形成基因 ylyA 编码一种新型的 RNA 聚合酶结合蛋白，该蛋白影响枯草芽孢杆菌中受晚期作用、孢子形成 σ(G) 因子控制的基因的表达。ylyA 突变体的孢子在萌发方面表现出缺陷，这对应于孢子萌发受体的膜水平的变化以及在萌发过程中调节二吡啶羧酸释放和孢子核心水合的蛋白质通道的变化。纯化的 YlyA 与 RNA 聚合酶相互作用，并刺激依赖 σ(G)但不依赖于管家 σ(A)因子的启动子的转录。YlyA 是一种以前未被识别的 RNA 聚合酶结合蛋白，专门用于调节参与孢子萌发的基因的表达。
