Zou Hong-Zhi, Zhao Yan-Qiu
Tumor Hospital Affiliated to Zhengzhou University, Zhengzhou, China.
Asian Pac J Cancer Prev. 2013;14(3):1769-72. doi: 10.7314/apjcp.2013.14.3.1769.
A case-control study of the association of miR-499A>G rs3746444 with risk of hepatocellular carcinoma (HCC)was conducted. Patients with HCC and healthy control subjects were recruited for genotyping of miR- 499A>G using duplex polymerase-chain-reaction with confronting-two-pair primer(PCR-RFLP) analysis. The MiR-499 GG genotype was associated with a decreased risk of HCC as compared with the miR-499 AA genotype (adjusted OR=0.74, 95%CI=0.24-0.96). Similarly, the GG genotype showed a 0.45-fold decreased HCC risk in a recessive model. The MiR-499 G allele was significantly associated with decreased risk of HCC among patients infected with HBV in a dominant model (OR=0.09, 95%CI= 0.02-0.29). In conclusion, the MiR-499A>G rs3746444 polymorphism is associated with HCC risk in the Chinese population, and may be useful predictive marker for CAD susceptibility.
开展了一项关于miR-499A>G rs3746444与肝细胞癌（HCC）风险相关性的病例对照研究。招募了HCC患者和健康对照者，采用双引物聚合酶链反应（PCR-RFLP）分析对miR-499A>G进行基因分型。与miR-499 AA基因型相比，miR-499 GG基因型与HCC风险降低相关（校正OR=0.74，95%CI=0.24-0.96）。同样，在隐性模型中，GG基因型的HCC风险降低了0.45倍。在显性模型中，miR-499 G等位基因与HBV感染患者的HCC风险降低显著相关（OR=0.09，95%CI=0.02-0.29）。总之，miR-499A>G rs3746444多态性与中国人群的HCC风险相关，可能是CAD易感性的有用预测标志物。
