Hao Yu-Xia, Wang Jun-Ping, Zhao Long-Feng
Department of Infection, The First of Hospital of Shanxi Medical University, Taiyuan, China E-mail :
Asian Pac J Cancer Prev. 2014 Jan;14(11):6601-4. doi: 10.7314/apjcp.2013.14.11.6601.
Associations between polymorphisms in miR-146aG>C, miR-196a2C>T and miR-499A>G and risk of HCC, and interaction with HBV infection in a Chinese population, were the target of the present research.
The duplex polymerase-chain-reaction with confronting-two-pair primers (PCR-RFLP) was performed to determine the genotypes of the miR-146aG>C, miR-196a2C>T and miR-499A>G genotypes. Associations of polymorphisms with the risk of HCC were estimated by conditional logistic regression analysis.
Drinking, family history of cancer, HBsAg and HCV were risk factors for HCC. Multivariate regression analyses showed that subjects carrying the miR-196a2 CC genotype had significantly increased risk of HCC, with an adjusted OR (95% CI) of 2.18 (1.23-3.80). In addition, cases carrying the miR-196a2 C allele had a 1.64-fold increase in the risk for HCC (95%CI=1.03-2.49). The miR-196a CT and TT genotypes greatly significantly increased the risk of HCC in subjects with HBV infection, with adjusted ORs (95% CI) of 2.02 (1.12-3.68) and 2.69 (1.28-5.71), respectively.
Our results demonstrate that miR-196a2 CC genotype and C allele have an important role in HCC risk in Chinese, especially in patients with HBV infection.
本研究旨在探讨中国人群中miR-146aG>C、miR-196a2C>T和miR-499A>G基因多态性与肝癌风险之间的关联，以及与乙肝病毒感染的相互作用。
采用双引物聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）来确定miR-146aG>C、miR-196a2C>T和miR-499A>G基因的基因型。通过条件逻辑回归分析评估多态性与肝癌风险的关联。
饮酒、癌症家族史、乙肝表面抗原（HBsAg）和丙肝病毒（HCV）是肝癌的危险因素。多变量回归分析显示，携带miR-196a2 CC基因型的受试者患肝癌的风险显著增加，校正后的比值比（95%置信区间）为2.18（1.23 - 3.80）。此外，携带miR-196a2 C等位基因的病例患肝癌的风险增加了1.64倍（9%置信区间=1.03 - 2.49）。miR-196a CT和TT基因型在乙肝病毒感染受试者中显著增加了患肝癌的风险，校正后的比值比（95%置信区间）分别为2.02（1.12 - 3.68）和2.69（1.28 - 5.71）。
我们的结果表明，miR-196a2 CC基因型和C等位基因在中国人群肝癌风险中起重要作用，尤其是在乙肝病毒感染患者中。
