Zhang Ji-Shun, Zhang Chuan, Yan Xue-Yan, Yuan Zhi-Fang, Duan Zhuo-Yang, Gao Hui
Department of Gastroenterology, the Affiliated Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
Asian Pac J Cancer Prev. 2013;14(3):1847-50. doi: 10.7314/apjcp.2013.14.3.1847.
We conducted a hospital case-control study by genotyping four potential functional single nucleotide polymorphisms (SNPs) to assess the association of Xeroderma pigmentosum complementation group F (XPF) with gastric cancer susceptibility, and role of XPF polymorphisms in combination with H.pylori infection in risk definition. A total of 331 patients with gastric cancer and 355 controls were collected. Four SNPs of XPF, rs180067, rs1799801, rs2276466 and rs744154, were genotyped by Taqman real-time PCR method with a 7900 HT sequence detector system. The gastric cancer patients were more likely to have smoking habit, a family history of cancer and H.pylori infection. We did not find any significant difference in the genotype distributions of XPF rs180067, rs1799801, rs2276466 and rs744154 between cases and controls. However, multivariate logistic analysis showed a non-significant decreased risk in patients carrying rs180067 G allele, rs1799801 T allele or rs2276466 T allele genotypes. A non-significant increased risk of gastric cancer was found in individuals carrying the rs744154 GG genotype. Stratification by H.pylori infection and smoking was not significantly different in polymorphisms of XPF rs180067, rs1799801, rs2276466 and rs744154. The four XPF SNPs did not show significant interaction with H.pylori infection and smoking status (P for interaction was 0.35 and 0.18, respectively). Our study indicated that polymorphisms in rs180067, rs1799801, rs2276466 and rs744154 may affect the risk of gastric cancer but further large sample size studies are needed to validate any association.
我们进行了一项医院病例对照研究，对四个潜在的功能性单核苷酸多态性（SNP）进行基因分型，以评估着色性干皮病F互补组（XPF）与胃癌易感性的关联，以及XPF多态性与幽门螺杆菌感染在风险定义中的联合作用。共收集了331例胃癌患者和355例对照。采用Taqman实时荧光定量PCR方法，利用7900 HT序列检测系统对XPF的四个SNP，即rs180067、rs1799801、rs2276466和rs744154进行基因分型。胃癌患者更有可能有吸烟习惯、癌症家族史和幽门螺杆菌感染。我们未发现病例组与对照组在XPF rs180067、rs1799801、rs2276466和rs744154的基因型分布上有任何显著差异。然而，多因素逻辑回归分析显示，携带rs180067 G等位基因、rs1799801 T等位基因或rs2276466 T等位基因基因型的患者风险有非显著降低。发现携带rs744154 GG基因型的个体患胃癌的风险有非显著增加。按幽门螺杆菌感染和吸烟进行分层后，XPF rs180067、rs1799801、rs2276466和rs744154的多态性无显著差异。这四个XPF SNP与幽门螺杆菌感染和吸烟状态未显示出显著的相互作用（相互作用的P值分别为0.35和0.18）。我们的研究表明，rs180067、rs1799801、rs2276466和rs744154的多态性可能影响胃癌风险，但需要进一步的大样本研究来验证任何关联。
