Division of Clinical Microbiology F 72, Karolinska Institutet, Karolinska University Hospital, Huddinge, SE 141 86 Stockholm, Sweden.
Diagn Microbiol Infect Dis. 2013 Jul;76(3):286-90. doi: 10.1016/j.diagmicrobio.2013.03.031. Epub 2013 May 13.
In the present study we investigated transport times for blood cultures from three tertiary-care hospitals to Karolinska University Laboratory and identified predictors of long transport times. Concomitantly, consequences of delayed incubation on total detection time (TDT) were analyzed by in vitro sepsis models. A total of 909 blood cultures were studied. The median (interquartile range) transport time was 9 (3-15) h. The hospital accommodating the microbiology laboratory had the shortest transport time compared to the other two hospitals (P < 0.0001). Samples taken between 16:00-24:00 had longer transport times compared to samples taken between 8:00-16:00 and 24:00-08:00 (P < 0.0001). In vitro experiments showed that TDT was longer for samples pre-incubated at room temperature (RT) for 19 h compared to the ones pre-incubated for 2 h or 9.5 h (P < 0.0001). In conclusion, off-site location, time of sampling and number of transports per day were related to, and predictors of transport time.
在本研究中，我们调查了来自三家三级保健医院的血培养物到卡罗林斯卡大学实验室的运输时间，并确定了运输时间延长的预测因素。同时，通过体外脓毒症模型分析了延迟孵育对总检测时间（TDT）的影响。共研究了 909 份血培养物。中位（四分位间距）运输时间为 9（3-15）小时。与其他两家医院相比，容纳微生物实验室的医院的运输时间最短（P < 0.0001）。与 8:00-16:00 和 24:00-08:00 之间采集的样本相比，16:00-24:00 采集的样本的运输时间更长（P < 0.0001）。体外实验表明，与预孵育 2 小时或 9.5 小时相比，预孵育 19 小时的样本 TDT 更长（P < 0.0001）。总之，场外位置、采样时间和每天的运输次数与运输时间有关，也是其预测因素。
