bioMérieux, Inc., Durham, NC, USA.
Eur J Clin Microbiol Infect Dis. 2021 Apr;40(4):699-705. doi: 10.1007/s10096-020-04042-z. Epub 2020 Oct 9.
Delayed entry of patient blood culture samples into a microbial detection system is unavoidable at times, due to off-shift staffing or transporting samples to centralized laboratories. Pre-incubation time and temperature of blood culture bottles are the most critical factors impacting recovery and detection of microorganisms. A total of 1377 BACT/ALERT® (BTA) Fastidious Antimicrobial Neutralization (FAN® PLUS) bottles (FA PLUS, FN PLUS, and PF PLUS) were tested after delayed entry times of 24 and 36 h at 20-25 °C (room temperature, RT) prior to loading into the BACT/ALERT® VIRTUO® microbial detection system (VIRTUO). Clinically relevant organisms were inoculated into bottles with 5-84 colony forming units (CFU) per bottle, and human blood (0 to 10 mL), and then loaded into the VIRTUO. When bottles were loaded without delay, a mean time to detection (TTD) of 9.6 h was observed. For delayed bottles, the TTD reported by the VIRTUO was added to the 24-h and 36-h delay times and resulted in average time to results of 32.5 h and 42.5 h, respectively. The FAN PLUS bottles in conjunction with the VIRTUO produced acceptable results when delays up to 24 h at 20-25 °C occur in loading.
由于轮班人员配置或将样本运送到集中实验室，有时不可避免地会延迟将患者血液培养样本输入微生物检测系统。血液培养瓶的预孵育时间和温度是影响微生物回收和检测的最关键因素。在将 BACT/ALERT® VIRTUO®微生物检测系统（VIRTUO）装入 BACT/ALERT® （BTA）Fastidious Antimicrobial Neutralization（FAN® PLUS）瓶（FA PLUS、FN PLUS 和 PF PLUS）之前，将其在 20-25°C（室温，RT）下延迟 24 和 36 小时后进行了总共 1377 次测试。将临床相关的生物体接种到每个瓶中有 5-84 个菌落形成单位（CFU）的瓶子中，并用人类血液（0 至 10 mL），然后将其装入 VIRTUO。当瓶子没有延迟地装入时，观察到平均检测时间（TTD）为 9.6 小时。对于延迟的瓶子，VIRTUO 报告的 TTD 被添加到 24 小时和 36 小时的延迟时间中，结果分别为 32.5 小时和 42.5 小时。当在 20-25°C 下发生长达 24 小时的延迟时，FAN PLUS 瓶与 VIRTUO 一起使用可产生可接受的结果。
