Chen Chen, Zhao Xiao-Hui, Yue Hui-Lan, Li Yu-Lin, Chen Tao
1Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, P. R. China.
J Chromatogr Sci. 2014 May-Jun;52(5):395-9. doi: 10.1093/chromsci/bmt048. Epub 2013 May 16.
An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenylpropanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascoside and 18 mg of isoacteoside, with purity values of 97 and 98%, respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition, the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 µg/mL, respectively.
建立了一种有效的高速逆流色谱法，用于从藏药长花马先蒿管状变种中制备分离和纯化两种苯丙素苷。采用氯仿-正丁醇-甲醇-水（4:3:4:5，v/v）组成的两相溶剂系统，对40 mg长花马先蒿管状变种提取物进行分离，得到20 mg毛蕊花糖苷和18 mg异毛蕊花糖苷，纯度分别为97%和98%。通过质子和碳核磁共振确定了这两种成分的化学结构。此外，采用2,2-二苯基-1-苦基肼（DPPH）-高效液相色谱法对两种苯丙素苷的抗氧化活性进行了评价，结果表明这两种成分表现出较强的抗氧化DPPH自由基清除活性，IC50值分别为15.6和18.9 μg/mL。
