Colarian J, Sethi A, Merchant A, Parikh N, Mutchnick M
Department of Medicine, Wayne State University School of Medicine, Detroit, MI.
Thymus. 1990 Jun;15(4):241-7.
Prostaglandins of the E series have been implicated as possible mediators of thymosin alpha 1 (TA-1) effect on mitogen stimulated lymphoproliferation. In the present study human peripheral blood mononuclear cells were stimulated with concanavalin A in the presence of TA-1, prostaglandin E2 and indomethacin individually, or in combination. Both TA-1 and indomethacin significantly enhanced the proliferative response over that seen with mitogen alone (less than 0.001). The magnitude of enhancement observed with indomethacin (20 microM) was considerably less than that of TA-1 and it was abolished with prostaglandin E2. The combination of indomethacin and TA-1 did not alter TA-1 effect and prostaglandin E2 (up to 250 ng/ml) did not abolish thymosin effect. These results suggest that TA-1 and prostaglandin E2 have opposite effects and that thymosin enhancement of mitogen induced proliferation is not mediated by prostaglandin E2.
E 系列前列腺素被认为可能是胸腺肽α1（TA-1）对有丝分裂原刺激的淋巴细胞增殖产生作用的介质。在本研究中，分别或联合使用 TA-1、前列腺素 E2 和吲哚美辛，在伴刀豆球蛋白 A 存在的情况下刺激人外周血单个核细胞。与单独使用有丝分裂原相比，TA-1 和吲哚美辛均显著增强了增殖反应（P<0.001）。观察到吲哚美辛（20μM）的增强幅度明显小于 TA-1，且前列腺素 E2 可消除该增强作用。吲哚美辛与 TA-1 的联合使用未改变 TA-1 的作用，且前列腺素 E2（高达 250 ng/ml）未消除胸腺肽的作用。这些结果表明，TA-1 和前列腺素 E2 具有相反的作用，且胸腺肽对有丝分裂原诱导增殖的增强作用不是由前列腺素 E2 介导的。
