Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA.
ACS Nano. 2013 Jun 25;7(6):5568-76. doi: 10.1021/nn401837q. Epub 2013 May 22.
It is still in high demand to develop extremely sensitive and accurate clinical tools for biomarkers of interest for early diagnosis and monitoring of diseases. In this report, we present a highly sensitive and compatible gold nanoparticle (AuNP)-based fluorescence-activatable probe for sensing ultralow levels of prostate-specific antigen (PSA) in patient serum samples. The limit of detection of the newly developed probe for PSA was pushed down to 0.032 pg/mL, which is more than 2 orders of magnitude lower than that of the conventional fluorescence probe. The ultrahigh sensitivity of this probe was attributed to the high loading efficiency of the dyes on AuNP surfaces and high fluorescence quenching-unquenching abilities of the dye-AuNP pairs. The efficiency and robustness of this probe were investigated in patient serum samples, demonstrating the great potential of this probe in real-world applications.
开发用于疾病早期诊断和监测的感兴趣生物标志物的极其敏感和准确的临床工具仍然有很高的需求。在本报告中，我们提出了一种基于金纳米粒子（AuNP）的高灵敏度和兼容的荧光可激活探针，用于检测患者血清样本中超低水平的前列腺特异性抗原（PSA）。新开发的 PSA 探针的检测限被推低至 0.032 pg/mL，比传统荧光探针低两个数量级以上。该探针的超高灵敏度归因于染料在 AuNP 表面上的高负载效率以及染料-AuNP 对的高荧光猝灭-解猝灭能力。在患者血清样本中对该探针的效率和稳健性进行了研究，表明该探针在实际应用中具有巨大的潜力。
