基于MoS@CuO-Au纳米复合材料的乐甫波表面声波免疫传感器用于实时超灵敏前列腺特异性抗原传感

Real-Time and Ultrasensitive Prostate-Specific Antigen Sensing Using Love-Mode Surface Acoustic Wave Immunosensor Based on MoS@CuO-Au Nanocomposites.

作者信息

Yu Yan, Xie Haiyu, Zhou Tao, Zhang Haonan, Lu Chenze, Tao Ran, Tang Zhaozhao, Luo Jingting

机构信息

Shenzhen Key Laboratory of Advanced Thin Films and Applications, GuangDong Engineering Technology Research Centre of Breath Test, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.

Key Laboratory of Specialty Agri-Products Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.

出版信息

Sensors (Basel). 2024 Nov 29;24(23):7636. doi: 10.3390/s24237636.

Prostate-specific antigen (PSA) is a well-established tumour marker for prostatic carcinoma. In this study, we present a novel, real-time, and ultrasensitive Love-mode surface acoustic wave (L-SAW) immunosensor for PSA detection enhanced by MoS@CuO-Au nanocomposite conjugation. The MoS@CuO-Au nanocomposites were analyzed by SEM, XRD, and EDS. The experiments show a significant improvement in sensitivity and detection limit compared with the previous detection methods utilizing nanogold alone to detect PSA biomolecules. The experimental results show a good linear relationship when the range of PSA concentrations between 200 pg/mL and 5 ng/mL was tested. The experimental results also show good specificity against alpha 1 fetoprotein and L-tryptophan disruptors.

前列腺特异性抗原（PSA）是一种已被广泛认可的前列腺癌肿瘤标志物。在本研究中，我们展示了一种新型、实时且超灵敏的洛夫波表面声波（L-SAW）免疫传感器，用于检测通过MoS@CuO-Au纳米复合材料共轭增强的PSA。通过扫描电子显微镜（SEM）、X射线衍射（XRD）和能谱分析（EDS）对MoS@CuO-Au纳米复合材料进行了分析。实验表明，与之前仅利用纳米金检测PSA生物分子的检测方法相比，该方法在灵敏度和检测限方面有显著提高。当测试PSA浓度范围在200 pg/mL至5 ng/mL之间时，实验结果显示出良好的线性关系。实验结果还表明，该传感器对甲胎蛋白和L-色氨酸干扰物具有良好的特异性。