Dipartimento di Bioscienze e Territorio, University of Molise, 86090 , Pesche, IS, Italy.
Physiol Plant. 2014 Feb;150(2):174-93. doi: 10.1111/ppl.12072. Epub 2013 Jun 13.
Temperate perennial woody plants use different environmental signals to coordinate their growth and development in relation to seasonal changes. Preliminary evidences suggest that, even during dormancy, plants maintain effective metabolic activities and molecular mechanisms ensuring them an eventual recording of mechanical loads during winter times. Despite their great importance for productivity and survival, plant biology investigations have poorly characterized the root growth cycle and its response to environmental stresses. In this study, we describe the proteomic changes occurring over the time in poplar root either in the absence or in response to a bending stress; corresponding expression of cell cycle regulator and auxin transporter genes was also evaluated by reverse transcription polymerase chain reaction analysis. Our results confirm previous evidences on the effect of the bending stress on the anticipation of root growth resumption, providing additional insights on a temporal modulation of various plant metabolic processes involved in dormancy break, growth resumption and stress response in the bent root; these events seem related to the differential compression and tension force distribution occurring over the plant taproot.
温带多年生木本植物利用不同的环境信号来协调其生长和发育与季节变化的关系。初步证据表明，即使在休眠期间，植物也保持有效的代谢活动和分子机制，以确保它们在冬季期间记录机械负荷。尽管它们对生产力和生存至关重要，但植物生物学研究对根生长周期及其对环境胁迫的反应特征描述很差。在这项研究中，我们描述了杨树根在没有或响应弯曲应力时随时间发生的蛋白质组变化；通过反转录聚合酶链反应分析还评估了细胞周期调节剂和生长素转运蛋白基因的对应表达。我们的结果证实了先前关于弯曲应激对根生长恢复预期的影响的证据，为休眠打破、生长恢复和弯曲根中的应激反应中涉及的各种植物代谢过程的时间调制提供了更多的见解；这些事件似乎与植物主根上发生的压缩和拉伸力分布的差异有关。
