Laboratorium voor Farmaceutische Analyse, Faculteit Farmaceutische Wetenschappen, KU Leuven, O&N2, PB-923, Herestraat 49, B-3000 Leuven, Belgium.
J Pharm Biomed Anal. 2014 Jan;87:142-54. doi: 10.1016/j.jpba.2013.04.015. Epub 2013 Apr 22.
This paper presents a general overview of the application of hydrophilic interaction chromatography (HILIC) in the analysis of antibiotics in different sample matrices including pharmaceutical, plasma, serum, fermentation broths, environmental water, animal origin, plant origin, etc. Specific applications of HILIC for analysis of aminoglycosides, β-lactams, tetracyclines and other antibiotics are reviewed. HILIC can be used as a valuable alternative LC mode for separating small polar compounds. Polar samples usually show good solubility in the mobile phase containing some water used in HILIC, which overcomes the drawbacks of the poor solubility often encountered in normal phase LC. HILIC is suitable for analyzing compounds in complex systems that elute near the void in reversed-phase chromatography. Ion-pair reagents are not required in HILIC which makes it convenient to couple with MS hence its increased popularity in recent years. In this review, the retention mechanism in HILIC is briefly discussed and a list of important applications is provided including main experimental conditions and a brief summary of the results. The references provide a comprehensive overview and insight into the application of HILIC in antibiotics analysis.
本文综述了亲水作用色谱（HILIC）在不同样品基质中抗生素分析的应用，包括药物、血浆、血清、发酵液、环境水、动物源性、植物源性等。综述了 HILIC 在氨基糖苷类、β-内酰胺类、四环素类等抗生素分析中的具体应用。HILIC 可作为一种有价值的替代 LC 模式，用于分离小极性化合物。极性样品通常在含有一定水的流动相中具有良好的溶解性，这克服了正相 LC 中经常遇到的溶解性差的缺点。HILIC 适用于分析反相色谱中在空隙附近洗脱的复杂体系中的化合物。HILIC 中不需要离子对试剂，这使其与 MS 连接更加方便，因此近年来越来越受欢迎。本文简要讨论了 HILIC 的保留机制，并提供了重要应用的列表，包括主要实验条件和简要结果总结。参考文献全面概述并深入了解了 HILIC 在抗生素分析中的应用。
