脊髓灰质炎病毒：突变体的产生与特性分析
Poliovirus: generation and characterization of mutants.
作者信息
Burrill Cecily P, Strings Vanessa R, Schulte Michael B, Andino Raul
机构信息
Department of Microbiology and Immunology, University of California, San Francisco, California.
出版信息
Curr Protoc Microbiol. 2013;Chapter 15:15H.2.1-15H.2.32. doi: 10.1002/9780471729259.mc15h02s29.
Poliovirus (PV) is the prototypical picornavirus. It is a non-enveloped RNA virus with a small (~7.5-kb) genome of positive polarity. cDNA clones of several strains are available, and infectious virus can be produced by the transfection of in vitro-transcribed viral genomes into an appropriate host cell. The ease of genetic studies in poliovirus is a primary reason that it has long served as a model to study RNA virus biology, pathogenesis, and evolution. Protocols for the generation and characterization of PV mutants are presented.
脊髓灰质炎病毒（PV）是典型的小核糖核酸病毒。它是一种无包膜的RNA病毒，基因组小（约7.5kb），呈正链极性。有几种毒株的cDNA克隆可供使用，通过将体外转录的病毒基因组转染到合适的宿主细胞中可产生感染性病毒。脊髓灰质炎病毒易于进行基因研究，这是它长期以来一直作为研究RNA病毒生物学、发病机制和进化的模型的主要原因。本文介绍了脊髓灰质炎病毒突变体的产生和鉴定方法。
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