脊髓灰质炎病毒：产生、定量、繁殖、纯化及储存
Poliovirus: generation, quantification, propagation, purification, and storage.
作者信息
Burrill Cecily P, Strings Vanessa R, Andino Raul
机构信息
Department of Microbiology and Immunology, University of California, San Francisco.
出版信息
Curr Protoc Microbiol. 2013;Chapter 15:15H.1.1-15H.1.27. doi: 10.1002/9780471729259.mc15h01s29.
Poliovirus (PV) is the prototypical picornavirus. It is a non-enveloped RNA virus with a small (~7.5-kb) genome of positive polarity. It has long served as a model to study RNA virus biology, pathogenesis, and evolution. cDNA clones of several strains are available, and infectious virus can be produced by the transfection of in vitro transcribed viral genomes into an appropriate host cell. PV infects many human and non-human primate cell lines including HeLa and HeLa S3 cells, and can grow to high titer in culture. Protocols for the production, propagation, quantification, and purification of PV are presented.
脊髓灰质炎病毒（PV）是典型的小核糖核酸病毒。它是一种无包膜的RNA病毒，基因组小（约7.5kb），呈正链极性。长期以来，它一直是研究RNA病毒生物学、发病机制和进化的模型。有几种毒株的cDNA克隆可供使用，通过将体外转录的病毒基因组转染到合适的宿主细胞中可产生感染性病毒。PV可感染许多人类和非人类灵长类细胞系，包括HeLa和HeLa S3细胞，并能在培养物中生长至高滴度。本文介绍了PV的生产、繁殖、定量和纯化方法。
Zotero 插件