利用可溶性脊髓灰质炎病毒受体敏化的磁性纳米粒子从粪便提取物中提取脊髓灰质炎病毒的方法的开发。
Development of poliovirus extraction method from stool extracts by using magnetic nanoparticles sensitized with soluble poliovirus receptor.
机构信息
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
出版信息
J Clin Microbiol. 2013 Aug;51(8):2717-20. doi: 10.1128/JCM.00499-13. Epub 2013 May 22.
Abstract
A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude biological samples.
摘要
一种从粪便提取物中提取脊髓灰质炎病毒（PV）的方法已经开发出来。用可溶性 PV 受体敏化的磁性纳米颗粒能够有效地从粪便提取物中提取 PV 假病毒（>99%的提取率）或内源性感染性 PV（>90%的提取率）。这种方法将有助于从粗生物样本中提取 PV。
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