Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
J Clin Microbiol. 2013 Aug;51(8):2717-20. doi: 10.1128/JCM.00499-13. Epub 2013 May 22.
A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude biological samples.
一种从粪便提取物中提取脊髓灰质炎病毒(PV)的方法已经开发出来。用可溶性 PV 受体敏化的磁性纳米颗粒能够有效地从粪便提取物中提取 PV 假病毒(>99%的提取率)或内源性感染性 PV(>90%的提取率)。这种方法将有助于从粗生物样本中提取 PV。