College of Biology and Science, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Carbohydr Polym. 2013 Jul 1;96(1):101-8. doi: 10.1016/j.carbpol.2013.03.072. Epub 2013 Mar 29.
Polysaccharides from Epimedium acuminatum were extracted by hot water and optimized with response surface methodology. The optimal conditions of the extraction were determined to be the ratio of water to raw material of 29.61, extraction temperature of 85.67°C and extraction time of 3.57 h. Under these optimal conditions, the yield of polysaccharide was 8.21%, which was well matched with the predictive yield (8.23%). Moreover, three purified fractions (EAP40-1, EAP60-1 and EAP80-2) were obtained for further chemical analysis, antioxidant activity analysis and antimicrobial activity analysis. EAP40-1 with molecular weight of 138,884 Da showed the best radical scavenging activity. Meanwhile, EAP60-1 with molecular weight of 114,667 Da was found to exhibit significant antihemolytic activity and antimicrobial activity.
从淫羊藿中提取多糖，采用响应面法优化。确定提取的最佳条件为水与原料比为 29.61，提取温度为 85.67°C，提取时间为 3.57 h。在这些最佳条件下，多糖得率为 8.21%，与预测产率（8.23%）吻合较好。此外，还得到了三种纯化级分（EAP40-1、EAP60-1 和 EAP80-2），用于进一步的化学分析、抗氧化活性分析和抗菌活性分析。分子量为 138884 Da 的 EAP40-1 表现出最好的自由基清除活性。同时，分子量为 114667 Da 的 EAP60-1 表现出显著的抗溶血活性和抗菌活性。
