College of Biology and Science, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Carbohydr Polym. 2013 Jan 30;92(1):63-8. doi: 10.1016/j.carbpol.2012.09.051. Epub 2012 Oct 2.
The polysaccharides were extracted from Epimedium acuminatum by hot water extraction, ultrasonic-assisted extraction, enzyme extraction, and microwave-assisted extraction. The physicochemical properties of Epimedium polysaccharides were then determined by chemical composition analysis, Fourier transform infrared (FT-IR) spectroscopy, and scanning electron microscopy (SEM) analysis. Further, the antioxidant activities were studied via different methods, including DPPH assay, ABTS assay, FRAP assay and AAPH-induced erythrocyte hemolysis assay. Results showed that the physicochemical properties of different polysaccharides were similar. Antioxidant assay indicated that four polysaccharides exhibited significant antioxidant activities in a dose-dependent manner. The antioxidant activities of the polysaccharides which obtained by hot water extraction were higher than those of other polysaccharides. Overall, E. acuminatum polysaccharides might be used as potential natural antioxidant.
水提法、超声辅助提取法、酶提取法和微波辅助提取法从淫羊藿中提取多糖。通过化学成分分析、傅里叶变换红外（FT-IR）光谱和扫描电子显微镜（SEM）分析测定淫羊藿多糖的理化性质。进一步通过 DPPH 法、ABTS 法、FRAP 法和 AAPH 诱导红细胞溶血法研究其抗氧化活性。结果表明，不同多糖的理化性质相似。抗氧化活性试验表明，四种多糖均具有剂量依赖性的显著抗氧化活性。热水提取得到的多糖的抗氧化活性高于其他多糖。总之，淫羊藿多糖可能作为潜在的天然抗氧化剂。
