Bidgoli Sepideh Arbabi, Mahdavi Moujan, Rezayat Seyed Mahdi, Korani Mitra, Amani Amir, Ziarati Parisa
Department of Toxicology & Pharmacology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
Acta Med Iran. 2013 May 7;51(4):203-8.
Antibiotic resistance to microorganisms is one of the major problems faced in the field of wound care in burns patients. Silver nanoparticles have come up as potent antimicrobial agent and are being evaluated in diverse medical applications ranging from silver based dressings to silver coated medical devices. We aimed in present study to test the release of nanosilver from nanosilver wound dressing and compare the dermal and systemic toxicity of nanosilver dressings in a repeated dose (21 days) model. Under general anesthesia, a limited standard 2nd degree burns were provided on the back of each rat in all treatment, negative control (simple dressing) and 5% silver nitrate groups, each contained 5 male wistar rats. According to the analysis made by atomic absorption spectrometry, the wound dressings released 0.599 ± 0.083 ppm of nanosilver during first 24 hrs of study. Daily observations were recoded and wounds were covered with new dressings each 24 hrs. Burn healing was observed in nanosilver wound dressing group in shorter time periods than the control groups. In toxicity assessment, this dressing didn't cause any hematological and histopathological abnormalities in treatment group but biochemical studies showed significant rise of plasma transaminase (ALT) at the endpoint (21 days) of the study (P=0.027). Portal mononuclear lymphoid and polymorphonuclear leukocyte infiltrations in three to four adjacent foci were recognized around the central hepatic vein in treatment group. Mild hepatotoxic effects of nanosilver wound dressing in wistar rat emphasize the necessity of more studies on toxicity potentials of low dose nanosilver by dermal applications.
微生物对抗生素的耐药性是烧伤患者伤口护理领域面临的主要问题之一。银纳米颗粒已成为一种有效的抗菌剂，正在从银基敷料到银涂层医疗器械等各种医疗应用中进行评估。我们在本研究中的目的是测试纳米银伤口敷料中纳米银的释放情况，并在重复给药（21天）模型中比较纳米银敷料的皮肤毒性和全身毒性。在全身麻醉下，在所有治疗组、阴性对照组（单纯敷料）和5%硝酸银组的每只大鼠背部造成有限的标准二度烧伤，每组包含5只雄性Wistar大鼠。根据原子吸收光谱法的分析，伤口敷料在研究的前24小时释放了0.599±0.083 ppm的纳米银。每天进行观察并每24小时用新敷料覆盖伤口。纳米银伤口敷料组的烧伤愈合时间比对照组短。在毒性评估中，该敷料在治疗组未引起任何血液学和组织病理学异常，但生化研究显示在研究终点（21天）时血浆转氨酶（ALT）显著升高（P=0.027）。在治疗组中央肝静脉周围三到四个相邻病灶处发现门静脉单核淋巴细胞和多形核白细胞浸润。纳米银伤口敷料对Wistar大鼠有轻度肝毒性作用，这强调了对低剂量纳米银经皮应用的毒性潜力进行更多研究的必要性。
