Heshmati Masomeh, ArbabiBidgoli Sepideh, Khoei Samideh, Rezayat Seyed Mahdi, Parivar Kazem
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
Iran J Pharm Res. 2015 Fall;14(4):1171-80.
Serious concerns have been expressed about potential health risks of Nano silver containing consumer products (AgNPs) therefore regulatory health risk assessment on such nanoparticles has become mandatory for the safe use of AgNPsinbiomedicalproducts with special concerns to the mutagenic potentials. In this study, we examined the inhibitory and mutagenicity effects of AgNPs in three different sizes of three colloidal AgNPs by Minimal Inhibitory concentration (MIC), Minimal Bactericidal Concentration (MBC) and Bacterial Reverse Mutation Assay (Ames test).All samples were characterized by transmission electron microscopy (TEM), X-Ray Diffraction (XRD) and Dynamic Light Scattering (DLS). DLS analysis showed lack of large agglomeration of the AgNPs and TEM results showed the spherical AgNPswith the average sizes of 15, 19.6, 21.8 nms. Furthermore the XRD analysis showed the crystalline samples with a face centered cubic structure of pure silver.AmestestresultsonColloidal silver nanoparticles showed lack of any mutation in TA100, TA98, YG1029S. typhymuriumstrains.In addition colloidal silver nanoparticles reduced the mutation ratesin all three strains in a concentration dependent manner .This finding creates a new issue in the possible antimutagenic effects of colloidal AgNPsas a new pharmaceutical productwhich should be consideredinfuture studiesby focusing onthephysicochemical properties of AgNPs.
人们对含纳米银的消费品（AgNPs）的潜在健康风险表示严重关切，因此，对这类纳米颗粒进行监管健康风险评估已成为安全使用AgNPs于生物医学产品的强制性要求，尤其要关注其致突变潜力。在本研究中，我们通过最小抑菌浓度（MIC）、最小杀菌浓度（MBC）和细菌回复突变试验（艾姆斯试验），研究了三种不同尺寸的三种胶体AgNPs的抑制和致突变作用。所有样品均通过透射电子显微镜（TEM）、X射线衍射（XRD）和动态光散射（DLS）进行表征。DLS分析表明AgNPs没有大的团聚，TEM结果显示为平均尺寸分别为15、19.6、21.8纳米的球形AgNPs。此外，XRD分析表明晶体样品具有纯银的面心立方结构。胶体银纳米颗粒的艾姆斯试验结果显示，TA100、TA98、YG1029鼠伤寒沙门氏菌菌株均未发生任何突变。此外，胶体银纳米颗粒以浓度依赖的方式降低了所有三种菌株的突变率。这一发现为胶体AgNPs作为一种新型药品可能具有的抗突变作用带来了一个新问题，在未来的研究中应通过关注AgNPs的物理化学性质来加以考虑。
