Plewka Andrzej, Madej Pawel, Plewka Danuta, Kowalczyk Anna, Miskiewicz Adam, Wittek Piotr, Leks Tomasz, Bilski Rafal
Department of Proteomics, Medical University of Silesia, Sosnowiec, Poland.
Folia Histochem Cytobiol. 2013;51(1):73-83. doi: 10.5603/FHC.2013.0011.
Uterine myomas represent one of the most frequently manifested benign tumors in women. They originate from smooth muscle cells of myometrium or its blood vessels. Many studies suggest that inflammation and pro-inflammatory factors may play a role in the carcinogenesis with an involvement of the transcription factor NF-kappaB which activity can be controlled by various environmental factors, including many cytokines. The aim of the study was to investigate the expression of NF-B, interleukin-1β (IL-1β), tumor necrosis factor a (TNF-α), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) in myometrium and uterine myomas of women of various age. The expression of NF-kappaB, selected cytokines and enzymes was estimated in women of reproductive or perimenopausal age by semiquantitative immunohistochemistry. The expression of the examined proteins was higher in myomas than in control myometrium and was dependent on the size of myomas and the age of women. However, the expression of the cytoplasmic NF-kappaB observed in uterine myomas was independent on the size of myomas and no significant differences were observed in the number of stained nuclei between control and myoma groups. Thus, the expression of proinflammatory factors in myomas was not accompanied by the nuclear activation of NF-kappaB p65. The results of our study indicate that the examined factors may be involved in the pathogenesis of benign tumors and not only malignant diseases.
子宫肌瘤是女性中最常见的良性肿瘤之一。它们起源于子宫肌层的平滑肌细胞或其血管。许多研究表明，炎症和促炎因子可能在致癌过程中发挥作用，其中转录因子核因子κB（NF-κB）参与其中，其活性可受多种环境因素控制，包括许多细胞因子。本研究的目的是调查不同年龄女性子宫肌层和子宫肌瘤中NF-κB、白细胞介素-1β（IL-1β）、肿瘤坏死因子α（TNF-α）、环氧化酶2（COX-2）和诱导型一氧化氮合酶（iNOS）的表达。通过半定量免疫组织化学法评估生育期或围绝经期女性中NF-κB、所选细胞因子和酶的表达。所检测蛋白质的表达在肌瘤中高于对照子宫肌层，并且取决于肌瘤大小和女性年龄。然而，在子宫肌瘤中观察到的细胞质NF-κB表达与肌瘤大小无关，对照和肌瘤组之间染色细胞核数量未观察到显著差异。因此，肌瘤中促炎因子的表达并未伴随NF-κB p65的核激活。我们的研究结果表明，所检测的因素可能参与良性肿瘤的发病机制，而不仅仅是恶性疾病。
