Department of International Health, Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, Denmark.
Contact Dermatitis. 2013 Jun;68(6):339-47. doi: 10.1111/cod.12043.
It has been suggested that interleukin (IL)-17 and IL-22 play important roles in the elicitation of human allergic contact dermatitis; however, the frequencies of T cell subtypes producing IL-17 and IL-22 in human allergic contact dermatitis are unknown.
To determine the frequencies of CD4(+) , CD8(+) and γδ T cells producing IL-17, IL-22 and interferon (IFN)-γ in the blood and skin from nickel-allergic patients.
PATIENTS/MATERIALS/METHODS: Blood samples were collected from 14 patients and 17 controls, and analysed by flow cytometry. Biopsies were taken from 5 patients and 6 controls, and analysed by immunohistochemistry and flow cytometry of skin lymphocytes.
We found an increased frequency of γδ T cells in the blood, but no differences in the distribution of cytokine-producing CLA(+) T cell subtypes in nickel-allergic patients as compared with controls. In nickel-allergic patients, there was massive cellular infiltration dominated by CD4(+) T cells producing IL-17, IL-22 and IFN-γ in nickel-challenged skin but not in vehicle-challenged skin.
CD4(+) T cells producing IL-17, IL-22 and IFN-γ are important effector cells in the eczematous reactions of nickel-induced allergic contact dermatitis in humans.
白细胞介素 (IL)-17 和 IL-22 被认为在人类变应性接触性皮炎的发病机制中发挥重要作用；然而，人类变应性接触性皮炎中产生 IL-17 和 IL-22 的 T 细胞亚群的频率尚不清楚。
确定镍过敏患者血液和皮肤中产生 IL-17、IL-22 和干扰素 (IFN)-γ 的 CD4(+)、CD8(+) 和 γδ T 细胞的频率。
患者/材料/方法：收集了 14 名患者和 17 名对照者的血液样本，并通过流式细胞术进行分析。对 5 名患者和 6 名对照者进行了活检，并通过免疫组织化学和皮肤淋巴细胞的流式细胞术进行了分析。
与对照组相比，我们发现镍过敏患者血液中 γδ T 细胞的频率增加，但产生细胞因子的 CLA(+)T 细胞亚群的分布无差异。在镍过敏患者中，在镍刺激的皮肤中存在大量以产生 IL-17、IL-22 和 IFN-γ 的 CD4(+)T 细胞为主的细胞浸润，但在载体刺激的皮肤中没有。
在人类镍诱导的变应性接触性皮炎的湿疹反应中，产生 IL-17、IL-22 和 IFN-γ 的 CD4(+)T 细胞是重要的效应细胞。
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