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低强度脉冲超声诱导脂肪干细胞成脂分化增强。

Low-intensity pulsed ultrasound induced enhanced adipogenesis of adipose-derived stem cells.

机构信息

State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China.

出版信息

Cell Prolif. 2013 Jun;46(3):312-9. doi: 10.1111/cpr.12031.

DOI:10.1111/cpr.12031
PMID:23692089
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6496813/
Abstract

OBJECTIVE

The aim of this study was to investigate effects of low-intensity pulsed ultrasound (LIPUS) on differentiation of adipose-derived stem cells (ASCs), in vitro.

MATERIALS AND METHODS

Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes PPAR-γ1, and APN, was examined by real-time PCR. Immunofluorescence (IF) staining was performed to test for PPAR-γ at the protein level.

RESULTS

Our data revealed that specific patterns of LIPUS up-regulated levels of both PPAR-γ1 and APN mRNA, and PPAR-γ protein.

CONCLUSIONS

In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis.

摘要

目的

本研究旨在探讨低强度脉冲超声（LIPUS）对体外脂肪干细胞（ASCs）分化的影响。

材料与方法

在诱导脂肪生成后立即或延迟 2 天，对小鼠 ASCs 进行 LIPUS 处理，持续 3 天或 5 天。通过实时 PCR 检测脂肪生成基因 PPAR-γ1 和 APN 的表达。通过免疫荧光（IF）染色检测 PPAR-γ 在蛋白质水平的表达。

结果

我们的数据显示，特定模式的 LIPUS 上调了 PPAR-γ1 和 APN mRNA 以及 PPAR-γ 蛋白的水平。

结论

在含有脂肪生成试剂的培养基中，LIPUS 增强了 ASC 的脂肪生成。

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本文引用的文献

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Multilineage differentiation of dental pulp stem cells from green fluorescent protein transgenic mice.绿色荧光蛋白转基因小鼠牙髓干细胞的多向分化。
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Compressive force inhibits adipogenesis through COX-2-mediated down-regulation of PPARgamma2 and C/EBPalpha.压缩力通过 COX-2 介导的 PPARγ2 和 C/EBPα 下调抑制脂肪生成。
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Experimental assessment of calvarial bone defect re-ossification stimulation using low-intensity pulsed ultrasound.使用低强度脉冲超声刺激颅骨骨缺损再骨化的实验评估
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Mechanical strain inhibits adipogenesis in mesenchymal stem cells by stimulating a durable beta-catenin signal.机械应变通过刺激持久的β-连环蛋白信号来抑制间充质干细胞的脂肪生成。
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