使用单光纤光学微束对哺乳动物细胞进行操作。
Manipulation of mammalian cells using a single-fiber optical microbeam.
作者信息
Mohanty Samarendra K, Mohanty Khyati S, Berns Michael W
机构信息
University of California-Irvine, Beckman Laser Institute, Irvine, California 92612, USA.
出版信息
J Biomed Opt. 2008 Sep-Oct;13(5):054049. doi: 10.1117/1.2983663.
Abstract
The short working distance of microscope objectives has severely restricted the application of optical micromanipulation techniques at larger depths. We show the first use of fiber-optic tweezers toward controlled guidance of neuronal growth cones and stretching of neurons. Further, by mode locking, the fiber-optic tweezers beam was converted to fiber-optic scissors, enabling dissection of neuronal processes and thus allowing study of the subsequent response of neurons to localized injury. At high average powers, lysis of a three-dimensionally trapped cell was accomplished.
摘要
显微镜物镜的短工作距离严重限制了光学微操纵技术在较深深度的应用。我们首次展示了使用光纤镊子对神经元生长锥进行可控引导以及对神经元进行拉伸。此外，通过锁模，光纤镊子光束被转换为光纤剪刀，能够对神经突进行切割，从而可以研究神经元对局部损伤的后续反应。在高平均功率下，实现了对三维捕获细胞的裂解。
相似文献
1
Manipulation of mammalian cells using a single-fiber optical microbeam.
J Biomed Opt. 2008 Sep-Oct;13(5):054049. doi: 10.1117/1.2983663.
2
Tilt control in optical tweezers.
J Biomed Opt. 2008 Jan-Feb;13(1):010503. doi: 10.1117/1.2870123.
3
The optical cell rotator.
Opt Express. 2008 Oct 13;16(21):16984-92. doi: 10.1364/oe.16.016984.
4
Combining multiple optical trapping with microflow manipulation for the rapid bioanalytics on microparticles in a chip.
Rev Sci Instrum. 2007 Nov;78(11):116101. doi: 10.1063/1.2804768.
5
Light forces the pace: optical manipulation for biophotonics.
J Biomed Opt. 2010 Jul-Aug;15(4):041503. doi: 10.1117/1.3475958.
6
Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery.
J Biomed Opt. 2008 Sep-Oct;13(5):050508. doi: 10.1117/1.2997375.
7
Investigation of inclined dual-fiber optical tweezers for 3D manipulation and force sensing.
Opt Express. 2009 Aug 3;17(16):13624-38. doi: 10.1364/oe.17.013624.
8
Multimodal biophotonic workstation for live cell analysis.
J Biophotonics. 2012 Jan;5(1):9-13. doi: 10.1002/jbio.201100052. Epub 2011 Aug 15.
9
Optical transfection using an endoscope-like system.
J Biomed Opt. 2011 Feb;16(2):028002. doi: 10.1117/1.3541781.
10
Spatially selecting a single cell for lysis using light-induced electric fields.
Small. 2014 Aug 13;10(15):3026-31. doi: 10.1002/smll.201400247. Epub 2014 Apr 9.
引用本文的文献
1
Reliable and mobile all-fiber modular optical tweezers.
Sci Rep. 2020 Nov 18;10(1):20099. doi: 10.1038/s41598-020-77067-1.
2
Optical Fiber Tweezers: A Versatile Tool for Optical Trapping and Manipulation.
Micromachines (Basel). 2020 Jan 21;11(2):114. doi: 10.3390/mi11020114.
3
Photo-Magnetic Irradiation-Mediated Multimodal Therapy of Neuroblastoma Cells Using a Cluster of Multifunctional Nanostructures.
Nanomaterials (Basel). 2018 Sep 29;8(10):774. doi: 10.3390/nano8100774.
4
Spatial temperature gradients guide axonal outgrowth.
Sci Rep. 2016 Jul 27;6:29876. doi: 10.1038/srep29876.
5
Optical Techniques in Optogenetics.
J Mod Opt. 2015;62(12):949-970. doi: 10.1080/09500340.2015.1010620.
6
Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis.
Biomed Opt Express. 2015 Mar 27;6(4):1512-9. doi: 10.1364/BOE.6.001512. eCollection 2015 Apr 1.
7
Crystalline magnetic carbon nanoparticle assisted photothermal delivery into cells using CW near-infrared laser beam.
Sci Rep. 2014 May 29;4:5106. doi: 10.1038/srep05106.
8
Highly effective photonic cue for repulsive axonal guidance.
PLoS One. 2014 Apr 9;9(4):e86292. doi: 10.1371/journal.pone.0086292. eCollection 2014.
9
Optofluidic realization and retaining of cell-cell contact using an abrupt tapered optical fibre.
Sci Rep. 2013;3:1993. doi: 10.1038/srep01993.
10
Low-intensity pulsed ultrasound induced enhanced adipogenesis of adipose-derived stem cells.
Cell Prolif. 2013 Jun;46(3):312-9. doi: 10.1111/cpr.12031.
本文引用的文献
1
Tapered fiber optical tweezers for microscopic particle trapping: fabrication and application.
Opt Express. 2006 Dec 11;14(25):12510-6. doi: 10.1364/oe.14.012510.
2
Manipulation and arrangement of biological and dielectric particles by a lensed fiber probe.
Opt Express. 2004 Aug 23;12(17):4123-8. doi: 10.1364/opex.12.004123.
3
Particle trapping in 3-D using a single fiber probe with an annular light distribution.
Opt Express. 2003 Oct 20;11(21):2775-82. doi: 10.1364/oe.11.002775.
4
In-depth activation of channelrhodopsin 2-sensitized excitable cells with high spatial resolution using two-photon excitation with a near-infrared laser microbeam.
Biophys J. 2008 Oct;95(8):3916-26. doi: 10.1529/biophysj.108.130187. Epub 2008 Jul 11.
5
A history of laser scissors (microbeams).
Methods Cell Biol. 2007;82:1-58. doi: 10.1016/S0091-679X(06)82001-7.
6
Optogenetics. Shining new light on neural circuits.
Science. 2006 Dec 15;314(5806):1674-6. doi: 10.1126/science.314.5806.1674.
7
Controlled induction, enhancement, and guidance of neuronal growth cones by use of line optical tweezers.
Opt Lett. 2005 Oct 1;30(19):2596-8. doi: 10.1364/ol.30.002596.
8
Neurosurgery: functional regeneration after laser axotomy.
Nature. 2004 Dec 16;432(7019):822. doi: 10.1038/432822a.
9
Self-rotation of red blood cells in optical tweezers: prospects for high throughput malaria diagnosis.
Biotechnol Lett. 2004 Jun;26(12):971-4. doi: 10.1023/b:bile.0000030041.94322.71.
10
Kinesin moves by an asymmetric hand-over-hand mechanism.
Science. 2003 Dec 19;302(5653):2130-4. doi: 10.1126/science.1092985. Epub 2003 Dec 4.
Zotero 插件