Mohanty Samarendra K, Mohanty Khyati S, Berns Michael W
University of California-Irvine, Beckman Laser Institute, Irvine, California 92612, USA.
J Biomed Opt. 2008 Sep-Oct;13(5):054049. doi: 10.1117/1.2983663.
The short working distance of microscope objectives has severely restricted the application of optical micromanipulation techniques at larger depths. We show the first use of fiber-optic tweezers toward controlled guidance of neuronal growth cones and stretching of neurons. Further, by mode locking, the fiber-optic tweezers beam was converted to fiber-optic scissors, enabling dissection of neuronal processes and thus allowing study of the subsequent response of neurons to localized injury. At high average powers, lysis of a three-dimensionally trapped cell was accomplished.
显微镜物镜的短工作距离严重限制了光学微操纵技术在较深深度的应用。我们首次展示了使用光纤镊子对神经元生长锥进行可控引导以及对神经元进行拉伸。此外，通过锁模，光纤镊子光束被转换为光纤剪刀，能够对神经突进行切割，从而可以研究神经元对局部损伤的后续反应。在高平均功率下，实现了对三维捕获细胞的裂解。
