Hertzman C M, Keipert P E, Chang T M
Int J Artif Organs. 1986 May;9(3):179-82.
Cross-linking hemoglobin (Hb) into Polyhemoglobin (PolyHb) for use as an artificial blood substitute may affect its antigenicity. To investigate this, male Sprague-Dawley rats are immunized with one of the following: rat stroma-free Hb (rSFHb), rat PolyHb (rPolyHb), human stroma-free Hb (hSFHb), and human PolyHb (hPolyHb). Antibody titers are quantified using a double antibody radioimmunoassay. These results show that more antibodies are produced to hPolyHb than to hSFHb, whereas rSFHB and rPolyHb are relatively non-antigenic. Thus, under homologous conditions, cross-linking hemoglobin does not significantly increase its antigenicity, whereas under heterologous conditions the molecule becomes more antigenic.
将血红蛋白（Hb）交联成多聚血红蛋白（PolyHb）用作人工血液替代品可能会影响其抗原性。为了对此进行研究，用以下物质之一对雄性斯普拉格 - 道利大鼠进行免疫：大鼠无基质血红蛋白（rSFHb）、大鼠多聚血红蛋白（rPolyHb）、人无基质血红蛋白（hSFHb）和人多聚血红蛋白（hPolyHb）。使用双抗体放射免疫测定法定量抗体滴度。这些结果表明，与hSFHb相比，针对hPolyHb产生的抗体更多，而rSFHB和rPolyHb相对无抗原性。因此，在同源条件下，交联血红蛋白不会显著增加其抗原性，而在异源条件下该分子变得更具抗原性。
