Tanigawa Yoshinori, Tanaka Manabu, Maeda Yoshinori, Nakagawachi Akira, Hamada Satsuki, Sakaguchi Yoshiro
Department of Anesthesiology and Critical Care Medicine, Faculty of Medicine, Saga University, Saga 849-850.
Masui. 2013 Apr;62(4):495-9.
Fresh frozen plasma (FFP) should be thawed in a water bath at 30-37 degrees C. Suitable temperature, the prevention for bacterial contamination, and the efficiency of the process are necessary for a thawing procedure. In this study, we compared the clotting factor activity and thawing time in different thawing procedures; a water bath, the thermostatic thawing chamber (FP-40, Hokuyo ; Kawasumi, Japan), and the microwave system (Transfusio-therm 2000 AMCO; Zeipel, Germany).
Thawing time and the clotting factor activity (prothrombin time: PT, prothrombin time-international normalized ratio: PT-INR, activated partial thromboplastin time: APTT, fibrinogen, andfactors V) of thawed FFP-5 units were measured.
Thawing time using Transfusio-therm 2000 was 11.4 minutes, which was faster than that using the water bath and FP-40 of about 39.5 and 27.3 minutes, respectively (P<0.01). There were no differences between the three methods in terms of the clotting factors.
The microwave system is useful in shortening the time safety, and maintaining the clotting factor activity in thawed FFP
新鲜冰冻血浆（FFP）应在30 - 37摄氏度的水浴中解冻。合适的温度、防止细菌污染以及解冻过程的效率对于解冻程序而言是必要的。在本研究中，我们比较了不同解冻程序（水浴、恒温解冻箱（FP - 40，北阳；日本川澄）和微波系统（Transfusio - therm 2000 AMCO；德国蔡佩尔））中的凝血因子活性和解冻时间。
测量解冻的5单位FFP的解冻时间和凝血因子活性（凝血酶原时间：PT、凝血酶原时间国际标准化比值：PT - INR、活化部分凝血活酶时间：APTT、纤维蛋白原和因子V）。
使用Transfusio - therm 2000的解冻时间为11.4分钟，分别比使用水浴和FP - 40的解冻时间快约39.5分钟和27.3分钟（P<0.01）。三种方法在凝血因子方面无差异。
微波系统有助于缩短解冻时间、保证安全并维持解冻后FFP中的凝血因子活性。
