Churchill W H, Schmidt B, Lindsey J, Greenberg M, Boudrow S, Brugnara C
Blood Bank, Brigham Women's Hospital, Boston, Massachusetts 02115.
Am J Clin Pathol. 1992 Feb;97(2):227-32. doi: 10.1093/ajcp/97.2.227.
We show in this report that fresh frozen plasma (FFP) can be thawed faster using a specifically designed microwave oven (MWO) (WesLabs Plasma Defroster, Westmorland Laboratories, Inc., New Brunswick, Canada) than using 37 degrees C water bath (WB) and that the thawed product was equivalent to FFP thawed by WB. Paired plasma bags (200 mL/bag) from plasma pools were frozen, stored at -35 degrees C, and thawed in parallel, one bag in MWO, the other in WB. Mean thaw time (mean + SD) by MWO was 6.99 + 1.3 minutes; by WB the time was 17.6 + 1.7 minutes (n = 24; P less than 0.005). Rapid calorimetry of thawed plasma showed that MWO-thawed FFP temperature was 20.4 + 2.5 degrees C, whereas WB-thawed FFP was 15.4 + 3.3 degrees C (n = 24; P less than 0.005). Except for thrombin time (MWO = 20.1 seconds; WB = 19.8 seconds; n = 24; P = 0.023), no significant differences were observed in the 23 other coagulation parameters and plasma proteins studied. Faster thawing and freedom from risk of contamination may make MWO the method of choice for emergency thawing of FFP.
我们在本报告中表明，使用专门设计的微波炉（MWO）（WesLabs血浆解冻器，加拿大新不伦瑞克省的Westmorland实验室公司）解冻新鲜冰冻血浆（FFP）比使用37摄氏度水浴（WB）解冻速度更快，且解冻后的产品与经WB解冻的FFP相当。从血浆库中取出成对的血浆袋（每袋200毫升）进行冷冻，储存在-35摄氏度，然后并行解冻，一袋用MWO解冻，另一袋用WB解冻。MWO的平均解冻时间（平均值±标准差）为6.99±1.3分钟；WB的解冻时间为17.6±1.7分钟（n = 24；P<0.005）。对解冻血浆进行快速量热法检测显示，MWO解冻的FFP温度为20.4±2.5摄氏度，而WB解冻的FFP温度为15.4±3.3摄氏度（n = 24；P<0.005）。在所研究的23项其他凝血参数和血浆蛋白中，除凝血酶时间外（MWO = 20.1秒；WB = 19.8秒；n = 24；P = 0.023），未观察到显著差异。更快的解冻速度以及无污染风险可能使MWO成为FFP紧急解冻的首选方法。
