Turner Matthew Alfred, Rahilly Louisa Jane, Katheryn O'Marra Shana
Emergency & Critical Care, Cape Cod Veterinary Specialists, Buzzards Bay, MA, 02532.
J Vet Emerg Crit Care (San Antonio). 2018 Nov;28(6):603-607. doi: 10.1111/vec.12768. Epub 2018 Oct 9.
Commercial microwave plasma defrosters (MPDs) are used globally in human medicine to safely thaw fresh-frozen plasma (FFP), but this technology has never been tested in a veterinary setting. This study was undertaken to assess the efficacy of a commercial MPD for the rapid thawing of canine FFP.
Twenty-three units (twelve 120 mL and eleven 240 mL) of canine FFP were thawed using an MPD. Time-to-thaw and pre- and postthawing temperatures of the units were measured. Clotting factor activities (factors II, V, VII, VIII, IX, X, and von Willebrand factor), fibrinogen concentrations, prothrombin times, and activated partial thromboplastin times were measured.
The evaluated MPD effectively thaws plasma quickly for both 120 mL units (2.7 ± 0.08 min) and 240 mL units (3.9 ± 0.15 min) while maintaining clinically relevant activities of clotting factors and fibrinogen concentration. While some measurements of factor VIII activity fell below the reference interval, none fell below 40%. One 240 mL unit had von Willebrand factor activity <70%. There was no evidence of excessively heated plasma to indicate a safety concern.
The MPD evaluated in this study provides a useful means to rapidly thaw canine FFP for correction of factor-deficient coagulopathy.
商用微波等离子体解冻器（MPD）在全球范围内被用于人类医学中安全解冻新鲜冰冻血浆（FFP），但该技术从未在兽医领域进行过测试。本研究旨在评估一种商用MPD对犬FFP快速解冻的效果。
使用MPD解冻23单位（12个120 mL和11个240 mL）犬FFP。测量各单位的解冻时间以及解冻前后的温度。检测凝血因子活性（因子II、V、VII、VIII、IX、X和血管性血友病因子）、纤维蛋白原浓度、凝血酶原时间和活化部分凝血活酶时间。
所评估的MPD能有效快速解冻血浆，120 mL单位血浆解冻时间为（2.7±0.08分钟），240 mL单位血浆解冻时间为（3.9±0.15分钟），同时维持凝血因子的临床相关活性和纤维蛋白原浓度。虽然一些因子VIII活性测量值低于参考区间，但均未低于40%。1个240 mL单位的血管性血友病因子活性<70%。没有证据表明血浆过度加热存在安全问题。
本研究中评估的MPD为快速解冻犬FFP以纠正因子缺乏性凝血病提供了一种有用的方法。
