Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
Langmuir. 2013 Jun 4;29(22):6645-51. doi: 10.1021/la400597e. Epub 2013 May 22.
The immobilization of biologically active species is crucial for the fabrication of smart bioactive surfaces. For this purpose, plasma polymerization is frequently used to modify the surface nature without affecting the bulk properties of the material. Thus, it is possible to create materials with surface functional groups that can promote the anchoring of all kinds of biomolecules. Different methodologies in protein immobilization have been developed in recent years, although some drawbacks are still not solved, such as the difficulties that some procedures involve and/or the denaturalization of the protein due to the immobilization process. In this work, two different strategies to covalently attach bovine serum albumin (BSA) protein are developed. Both techniques are compared in order to understand how the nature of the surface modification affects the conformation of the protein upon immobilization.
生物活性物质的固定化对于智能生物活性表面的制备至关重要。为此，常采用等离子体聚合来修饰表面性质，而不影响材料的体相性质。因此，有可能制造出具有表面功能基团的材料，这些基团可以促进各种生物分子的锚定。近年来已经开发出了不同的蛋白质固定化方法，但仍存在一些尚未解决的缺点，例如一些步骤涉及的困难和/或由于固定化过程导致蛋白质的变性。在这项工作中，开发了两种共价固定牛血清白蛋白（BSA）蛋白的不同策略。为了了解表面修饰的性质如何影响固定化过程中蛋白质的构象，对这两种技术进行了比较。
