College of Chemistry, Nankai University, Tianjin 300071, China.
Sensors (Basel). 2013 Apr 25;13(5):5493-506. doi: 10.3390/s130505493.
The molecularly imprinted polymer based on polypyrrole film with incorporated graphene oxide was fabricated and used for electrochemical determination of quercetin. The electrochemical behavior of quercetin on the modified electrode was studied in detail using differential pulse voltammetry. The oxidation peak current of quercetin in B-R buffer solution (pH = 3.5) at the modified electrode was regressed with the concentration in the range from 6.0 × 10(-7) to 1.5 × 10(-5) mol/L (r2 = 0.997) with a detection limit of 4.8 × 10(-8) mol/L (S/N = 3). This electrode showed good stability and reproducibility. In the above mentioned range, rutin or morin which has similar structures and at the same concentration as quercetin did not interfere with the determination of quercetin. The applicability of the method for complex matrix analysis was also evaluated.
基于聚吡咯薄膜的分子印迹聚合物与氧化石墨烯复合，被制备并用于电化学测定槲皮素。使用差分脉冲伏安法详细研究了修饰电极上槲皮素的电化学行为。在修饰电极的 B-R 缓冲溶液（pH = 3.5）中，槲皮素的氧化峰电流与浓度在 6.0×10(-7)至 1.5×10(-5) mol/L 范围内呈线性关系（r2 = 0.997），检测限为 4.8×10(-8) mol/L（S/N = 3）。该电极具有良好的稳定性和重现性。在上述范围内，与槲皮素结构相似且浓度相同的芦丁或杨梅素不会干扰槲皮素的测定。该方法对复杂基质分析的适用性也进行了评估。
