Institute of Gallbladder Disease of Panyu, Guangzhou, People's Republic of China.
J Clin Microbiol. 2013 Aug;51(8):2551-5. doi: 10.1128/JCM.00946-13. Epub 2013 May 22.
To improve the rate of detection of Clonorchis sinensis infection, we compared different specimens from patients with cholecystolithiasis. Feces, gallbladder bile, and gallbladder stones collected from 179 consecutive patients with cholecystolithiasis underwent microscopic examination, and according to the results, 30 egg-positive and 30 egg-negative fecal, gallbladder bile, and gallbladder stone specimens, respectively, underwent real-time fluorescent PCR. The detection rates of eggs in feces, bile, and gallbladder stones were 30.7%, 44.7%, and 69.8%, respectively, and the differences were statistically significant (P<0.01). The PCR results confirmed that the eggs in the specimens were C. sinensis eggs. Eggs in the feces were "fresh" and in the gallbladder stones were "old." Microscopic examination of gallbladder stones may improve the detection rates of C. sinensis infection, which is important for developing individualized treatments to prevent the recurrence of gallbladder stones and to prevent the occurrence of severe liver damage and cholangiocarcinoma.
为了提高华支睾吸虫感染的检出率，我们比较了胆石症患者的不同标本。对 179 例连续胆石症患者的粪便、胆囊胆汁和胆囊结石进行了显微镜检查，并根据检查结果，分别对 30 份粪便阳性和 30 份粪便阴性、胆囊胆汁和胆囊结石进行了实时荧光 PCR 检测。粪便、胆汁和胆囊结石中虫卵的检出率分别为 30.7%、44.7%和 69.8%，差异有统计学意义（P<0.01）。PCR 结果证实，这些标本中的虫卵为华支睾吸虫卵。粪便中的虫卵是“新鲜的”，而胆囊结石中的虫卵是“陈旧的”。胆囊结石的显微镜检查可能会提高华支睾吸虫感染的检出率，这对于制定个体化治疗方案以预防胆囊结石复发和防止严重肝损伤和胆管癌的发生非常重要。
