Qiao Tie, Ma Rui-hong, Luo Zhen-liang, Yang Liu-qing, Luo Xiao-bing, Zheng Pei-ming
The Second People's Hospital of Panyu, Panyu, Guangzhou 511430, Guangdong Province, PR China; Institute of Gallbladder Disease of Panyu, Guangzhou 511430, Guangdong Province, PR China.
The Sixth People's Hospital of Nansha, Nansha, Guangzhou 511470, Guangdong Province, PR China.
Acta Trop. 2014 Oct;138:28-37. doi: 10.1016/j.actatropica.2014.06.004. Epub 2014 Jun 16.
Calcium carbonate gallbladder stones were easily neglected because they were previously reported as a rare stone type in adults. The aim of this study was to investigate the relationship between calcium carbonate stones and Clonorchis sinensis infection. A total of 598 gallbladder stones were studied. The stone types were identified by FTIR spectroscopy. The C. sinensis eggs and DNA were detected by microscopic examination and real-time fluorescent PCR respectively. And then, some egg-positive stones were randomly selected for further SEM examination. Corresponding clinical characteristics of patients with different types of stones were also statistically analyzed. The detection rate of C. sinensis eggs in calcium carbonate stone, pigment stone, mixed stone and cholesterol stone types, as well as other stone types was 60%, 44%, 36%, 6% and 30%, respectively, which was highest in calcium carbonate stone yet lowest in cholesterol stone. A total of 182 stones were egg-positive, 67 (37%) of which were calcium carbonate stones. The C. sinensis eggs were found adherent to calcium carbonate crystals by both light microscopy and scanning electron microscopy. Patients with calcium carbonate stones were mainly male between the ages of 30 and 60, the CO2 combining power of patients with calcium carbonate stones were higher than those with cholesterol stones. Calcium carbonate gallbladder stones are not rare, the formation of which may be associated with C. sinensis infection.
碳酸钙胆囊结石很容易被忽视，因为它们以前在成人中被报道为一种罕见的结石类型。本研究的目的是探讨碳酸钙结石与华支睾吸虫感染之间的关系。共研究了598颗胆囊结石。通过傅里叶变换红外光谱法鉴定结石类型。分别通过显微镜检查和实时荧光定量PCR检测华支睾吸虫卵和DNA。然后，随机选择一些虫卵阳性的结石进行进一步的扫描电镜检查。还对不同类型结石患者的相应临床特征进行了统计分析。碳酸钙结石、色素结石、混合结石、胆固醇结石以及其他结石类型中华支睾吸虫卵的检出率分别为60%、44%、36%、6%和30%，其中碳酸钙结石中最高，胆固醇结石中最低。共有182颗结石虫卵阳性，其中67颗（37%）为碳酸钙结石。通过光学显微镜和扫描电子显微镜均发现华支睾吸虫卵附着于碳酸钙晶体上。碳酸钙结石患者以30至60岁男性为主，碳酸钙结石患者的二氧化碳结合力高于胆固醇结石患者。碳酸钙胆囊结石并不罕见，其形成可能与华支睾吸虫感染有关。
