Lin Tien-Huang, Tan Tzu-Wei, Tsai Tsung-Hsun, Chen Chi-Cheng, Hsieh Teng-Fu, Lee Shang-Sen, Liu Hsin-Ho, Chen Wen-Chi, Tang Chih-Hsin
School of Chinese Medicine, China Medical University, Taichung 404, Taiwan.
Int J Mol Sci. 2013 May 8;14(5):9790-802. doi: 10.3390/ijms14059790.
Prostate cancer is the most commonly diagnosed malignancy in men and shows a predilection for metastasis to the bone. D-pinitol, a 3-methoxy analogue of d-chiro-inositol, was identified as an active principle in soy foods and legumes, and it has been proven to induce tumor apoptosis and metastasis of cancer cells. In this study, we investigated the anti-metastasis effects of D-pinitol in human prostate cancer cells. We found that D-pinitol reduced the migration and the invasion of prostate cancer cells (PC3 and DU145) at noncytotoxic concentrations. Integrins are the major adhesive molecules in mammalian cells and have been associated with the metastasis of cancer cells. Treatment of prostate cancer cells with D-pinitol reduced mRNA and cell surface expression of αvβ3 integrin. In addition, D-pinitol exerted its inhibitory effects by reducing focal adhesion kinase (FAK) phosphorylation, c-Src kinase activity and NF-kB activation. Thus, D-pinitol may be a novel anti-metastasis agent for the treatment of prostate cancer metastasis.
前列腺癌是男性中最常被诊断出的恶性肿瘤，且易发生骨转移。D-松醇是D-手性肌醇的3-甲氧基类似物，被确定为大豆食品和豆类中的一种活性成分，并且已被证明可诱导肿瘤细胞凋亡和癌细胞转移。在本研究中，我们调查了D-松醇对人前列腺癌细胞的抗转移作用。我们发现，在无细胞毒性浓度下，D-松醇可降低前列腺癌细胞（PC3和DU145）的迁移和侵袭能力。整合素是哺乳动物细胞中的主要黏附分子，与癌细胞转移有关。用D-松醇处理前列腺癌细胞可降低αvβ3整合素的mRNA和细胞表面表达。此外，D-松醇通过降低粘着斑激酶（FAK）磷酸化、c-Src激酶活性和NF-κB激活发挥其抑制作用。因此，D-松醇可能是一种用于治疗前列腺癌转移的新型抗转移药物。
