Nanda I, Deubelbeiss C, Guttenbach M, Epplen J T, Schmid M
Institut für Humangenetik der Universität, Würzburg, Federal Republic of Germany.
Hum Genet. 1990 Jul;85(2):187-94. doi: 10.1007/BF00193194.
Tandemly organized simple repetitive sequences are widespread in all eukaryotes. The organization of the simple tetrameric (GACA)n sequences at chromosomal loci has been investigated using in situ hybridization with chemically pure oligonucleotide probes. Both biotin- and digoxigenin-attached (GACA)4 probes reveal specific hybridization signals over the short arms of all acrocentric autosomes in man. In the other examined primates the NOR-bearing autosomes could be detected by in situ hybridization with (GACA)4, and a major concentration of the GACA simple repeats could be observed on the Y chromosome in the gibbon and mouse: the hybridization site in the gibbon Y chromosome coincides particularly with the silver-stainable NOR. In the past, accumulations of (GACA)n sequences were demonstrated mainly on vertebrate sex chromosomes. Therefore, the organization of GACA simple sequences is discussed in the context of their evolutionary potential accumulation and the possible linkage with the primate rDNA loci.
串联排列的简单重复序列在所有真核生物中广泛存在。已使用化学纯寡核苷酸探针进行原位杂交，研究了染色体位点上简单四聚体(GACA)n序列的组织情况。生物素和地高辛标记的(GACA)4探针均在人类所有近端着丝粒常染色体的短臂上显示出特异性杂交信号。在其他被检测的灵长类动物中，通过与(GACA)4进行原位杂交可检测到携带核仁组织区的常染色体，并且在长臂猿和小鼠的Y染色体上可观察到GACA简单重复序列的主要集中区域：长臂猿Y染色体上的杂交位点特别与可银染的核仁组织区重合。过去，(GACA)n序列的积累主要在脊椎动物性染色体上得到证实。因此，在GACA简单序列进化上的潜在积累及其与灵长类动物核糖体DNA位点可能的联系的背景下，对其组织情况进行了讨论。
