Gortner G, Nenno M, Weising K, Zink D, Nagl W, Kahl G
Plant Molecular Biology, Biocentre, University of Frankfurt, Frankfurt/Main, Germany.
Chromosome Res. 1998 Feb;6(2):97-104. doi: 10.1023/a:1009282828236.
We used fluorescence in situ hybridization to probe the physical organization of five simple sequence repeat motifs and the Arabidopsis-type telomeric repeat in metaphase chromosomes and interphase nuclei of chickpea (Cicer arietinum L.). Hybridization signals were observed with the whole set of probes and on all chromosomes, but the distribution and intensity of signals varied depending on the motif. On root-tip metaphase chromosomes, CA and GATA repeats were mainly restricted to centromeric areas, with additional GATA signals along some chromosomes. TA, A and AAC repeats were organized in a more dispersed manner, with centromeric regions being largely excluded. In interphase nuclei of the inner integument, CA and GATA signals predominantly occurred in the heterochromatic endochromocentres, whereas the other motifs were found both in eu- and heterochromatin. The distribution of the Arabidopsis-type telomeric repeat (TTTAGGG)n on metaphase chromosomes was found to be quite exceptional. One major cluster of repeats was spread along the short arm of chromosome B, whereas a second, weaker signal occurred interstitially on chromosome A. Only faint and inconsistent hybridization signals were visualized with the same probe at the chromosomal termini.
我们使用荧光原位杂交技术，在鹰嘴豆（Cicer arietinum L.）的中期染色体和间期细胞核中，探测五个简单序列重复基序以及拟南芥型端粒重复序列的物理组织情况。使用全套探针在所有染色体上均观察到杂交信号，但信号的分布和强度因基序而异。在根尖中期染色体上，CA和GATA重复序列主要局限于着丝粒区域，沿部分染色体还有额外的GATA信号。TA、A和AAC重复序列的分布更为分散，着丝粒区域基本没有信号。在内珠被的间期细胞核中，CA和GATA信号主要出现在异染色质内染色中心，而其他基序在常染色质和异染色质中均有发现。拟南芥型端粒重复序列（TTTAGGG）n在中期染色体上的分布相当特殊。一组主要的重复序列簇沿着B染色体的短臂分布，而在A染色体上有第二个较弱的信号出现在中间位置。使用同一探针在染色体末端仅观察到微弱且不一致的杂交信号。
