Phasomkusolsil Siriporn, Tawong Jaruwan, Monkanna Nantaporn, Pantuwatana Kanchana, Damdangdee Nuttapon, Khongtak Weeraphan, Kertmanee Yossasin, Evans Brian P, Schuster Anthony L
Department of Entomology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand.
J Vector Ecol. 2013 Jun;38(1):38-45. doi: 10.1111/j.1948-7134.2013.12006.x.
Artificial membrane-feeding techniques have replaced direct feeding on animals for the maintenance of malaria and arbovirus vectors in many laboratories. Membrane feeding facilitates controlled experimentation of pathogen transmission during mosquito feeding. Sheep blood is commonly used due to its availability and low cost. We evaluated the impact of blood source (human, guinea pig, sheep, and hamster via direct feeding) on feeding rates, adult survival, fecundity, hatching rates, and developmental times for five species of laboratory-colonized mosquitoes (Anopheles dirus, An. cracens, An. minimus, An. sawadwongporni, and Ae. aegypti). We found that feeding rates differ among blood sources within mosquito species. Survival, fecundity, and hatching rates were lower in all Anopheles species and Ae. aegypti after membrane feeding on sheep blood. Survival rates seven days post-feeding on sheep blood were significantly lower (P<0.05) for An. dirus (84.2%), An. minimus (67.2%), An. sawadwongporni (51.5%), and An. cracens (35.5%) relative to other blood sources. An. minimus and An. sawadwongporni laid no eggs by seven days post-feeding with sheep blood, while An. dirus and An. cracens produced significantly fewer numbers of eggs and demonstrated significantly lower hatching rates relative to what was observed with the other blood sources. These findings support the conclusion that sheep blood is not a suitable blood source for laboratory rearing of Anopheles spp.
在许多实验室中，人工膜饲技术已取代直接喂饲动物来维持疟疾病媒和虫媒病毒病媒。膜饲有助于在蚊子取食期间对病原体传播进行可控实验。由于绵羊血易于获取且成本低廉，所以它是常用的血液来源。我们评估了血液来源（通过直接喂饲获取的人血、豚鼠血、绵羊血和仓鼠血）对五种实验室饲养的蚊子（大劣按蚊、克劳按蚊、微小按蚊、萨氏按蚊和埃及伊蚊）的取食率、成虫存活率、繁殖力、孵化率和发育时间的影响。我们发现，在蚊子种类中，不同血液来源的取食率存在差异。所有按蚊种类和埃及伊蚊在以绵羊血进行膜饲后，其存活率、繁殖力和孵化率均较低。相对于其他血液来源，大劣按蚊（84.2%）、微小按蚊（67.2%）、萨氏按蚊（51.5%）和克劳按蚊（35.5%）在以绵羊血喂饲七天后的存活率显著较低（P<0.05）。微小按蚊和萨氏按蚊在以绵羊血喂饲七天后未产卵，而大劣按蚊和克劳按蚊相对于其他血液来源产下的卵数量显著减少，且孵化率显著较低。这些发现支持了以下结论：绵羊血不是实验室饲养按蚊属的合适血液来源。
