Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, IHMT-NOVA, Rua da Junqueira 100, 1349-008, Lisboa, Portugal.
Sci Rep. 2024 Aug 22;14(1):19473. doi: 10.1038/s41598-024-70090-6.
Mosquito-borne diseases kill millions of people each year. Therefore, many innovative research and population control strategies are being implemented but, most of them require large-scale production of mosquitoes. Mosquito rearing depends on fresh blood from human donors, experimentation animals or slaughterhouses, which constitutes a strong drawback since high blood quantities are needed, raising ethical and financial constraints. To eliminate blood dependency and the use of experimentation animals, we previously developed BLOODless, a patented diet that represents an important advance towards sustainable mosquito breeding in captivity. BLOODless diet was used to maintain a colony of Anopheles stephensi for 40 generations. Bloodmeal appetite, fitness, Plasmodium berghei infectivity, whole genome sequencing and microbiota were evaluated over time. Here we show that BLOODless can be implemented in Anopheles insectaries since it allows long-term rearing of mosquitoes in captivity, without a detectable effect on their fitness, infectivity, nor on their midgut and salivary microbiota composition.
每年有数百万人死于蚊媒疾病。因此，许多创新性的研究和人口控制策略正在实施，但其中大多数都需要大规模生产蚊子。蚊子的饲养依赖于人类供体、实验动物或屠宰场的新鲜血液，这是一个很大的缺点，因为需要大量的血液，这带来了伦理和财务方面的限制。为了消除对血液的依赖和实验动物的使用，我们之前开发了 BLOODless，一种专利饮食，这是朝着可持续的圈养蚊子繁殖迈出的重要一步。我们曾用 BLOODless 饮食来维持 40 代的斯氏按蚊种群。随着时间的推移，评估了血粉食欲、适应性、疟原虫伯氏疟原虫感染力、全基因组测序和微生物群。在这里，我们表明 BLOODless 可以在按蚊饲养场实施，因为它可以在没有明显影响其适应性、感染力、中肠和唾液微生物群组成的情况下，允许蚊子在圈养中长期繁殖。
