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一种便捷的用于酵母鉴定的微量滴定板方法。

A convenient microtitre tray procedure for yeast identification.

作者信息

Heard G M, Fleet G H

机构信息

Department of Food Science and Technology, University of New South Wales, Kensington, Australia.

出版信息

J Appl Bacteriol. 1990 May;68(5):447-51. doi: 10.1111/j.1365-2672.1990.tb02895.x.

Abstract

Media for yeast identification tests were incorporated into the wells of a microtitre tray. The tests included fermentation and assimilation of carbohydrates, assimilation of nitrogen compounds, growth in vitamin-free medium, resistance to cycloheximide, and observations for cell morphology and sporulation. Results of tests conducted in the trays showed very good agreement with those obtained by conventional methods. Eighteen reference yeasts were correctly identified from tests conducted in the trays. The trays of media could be stored, and provided a convenient system for yeast identification.

摘要

用于酵母鉴定试验的培养基被加入到微量滴定板的孔中。这些试验包括碳水化合物的发酵和同化、含氮化合物的同化、在无维生素培养基中的生长、对放线菌酮的抗性,以及细胞形态和孢子形成的观察。在滴定板中进行的试验结果与通过传统方法获得的结果非常吻合。从滴定板中进行的试验中正确鉴定出了18种参考酵母。培养基滴定板可以储存,为酵母鉴定提供了一个方便的系统。

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