Kali A, Srirangaraj S, Charles P M V
Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Pondicherry, India.
Indian J Med Microbiol. 2015 Apr-Jun;33(2):293-5. doi: 10.4103/0255-0857.154884.
Carbohydrate fermentation test is a well-established technique for speciation of bacteria and fungi. However, long incubation period, cost and procedural complexity may limit its use. Here, we describe a simple modification of conventional carbohydrate fermentation technique using microtitre plate. Thirty-one yeast isolates were identified based on their fermentation property by microtitre plate method and results were compared with conventional method. The modified method had 85.7% sensitivity and 100% specificity. The average time taken to produce positive reaction was 24 hours. When compared with conventional method, modified method reduced turn-around time and cost of processing without significant increase in discordant results.
碳水化合物发酵试验是一种成熟的用于细菌和真菌分类的技术。然而，较长的孵育期、成本和操作复杂性可能会限制其应用。在此，我们描述了一种使用微量滴定板对传统碳水化合物发酵技术进行的简单改良。通过微量滴定板法根据31株酵母分离株的发酵特性进行鉴定，并将结果与传统方法进行比较。改良后的方法灵敏度为85.7%，特异性为100%。产生阳性反应的平均时间为24小时。与传统方法相比，改良后的方法缩短了周转时间和处理成本，且不一致结果没有显著增加。
