Lewis S, Locker A, Todd J H, Bell J A, Nicholson R, Elston C W, Blamey R W, Ellis I O
Department of Histopathology, City Hospital, Nottingham.
J Clin Pathol. 1990 May;43(5):385-9. doi: 10.1136/jcp.43.5.385.
A series of 90 patients with primary operable breast cancer and with up to 36 months of follow up were investigated for expression of epidermal growth factor receptor (EGFR) in their tumours by immunocytochemical staining with the monoclonal antibody EGFR 1. Tumour samples were snap frozen in liquid nitrogen immediately after resection and subsequently stained using a standard indirect immunocytochemical method. Tumour staining was assessed by two observers and scored on a four point scale (0-3). Thirteen (14%) tumours showed positive immunoreactivity. A strong correlation between distinct EGFR expression and short disease free interval was observed. Significant correlations were also shown with oestrogen and progesterone receptor expression and tumour nuclear size. No significant association was found with tumour size, lymph node stage, and histological grade. The association with disease free interval remained significant in multivariate analysis.
对90例原发性可手术乳腺癌患者进行了一系列研究，这些患者的随访时间长达36个月，通过用单克隆抗体EGFR 1进行免疫细胞化学染色来检测其肿瘤中表皮生长因子受体（EGFR）的表达。肿瘤样本在切除后立即在液氮中速冻，随后采用标准间接免疫细胞化学方法进行染色。由两名观察者对肿瘤染色进行评估，并按四点量表（0 - 3）评分。13例（14%）肿瘤显示阳性免疫反应性。观察到EGFR的不同表达与无病生存期短之间存在强相关性。还显示与雌激素和孕激素受体表达以及肿瘤核大小存在显著相关性。未发现与肿瘤大小、淋巴结分期和组织学分级有显著关联。在多变量分析中，与无病生存期的关联仍然显著。
