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萝卜（Raphanus sativus L. 叶）乙醇提取物抑制 MDA-MB-231 人乳腺癌细胞中 ErbB(2) 和 ErbB(3) 的蛋白和 mRNA 表达。

Radish (Raphanus sativus L. leaf) ethanol extract inhibits protein and mRNA expression of ErbB(2) and ErbB(3) in MDA-MB-231 human breast cancer cells.

机构信息

Department of Food Science and Nutrition, Dankook University, Gyeonggi 448-701, Korea.

出版信息

Nutr Res Pract. 2011 Aug;5(4):288-93. doi: 10.4162/nrp.2011.5.4.288. Epub 2011 Aug 31.

DOI:10.4162/nrp.2011.5.4.288
PMID:21994522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3180678/
Abstract

In this study, we investigated the effects of the ethanol extract of aerial parts of Raphanus sativus L. (ERL) on breast cancer cell proliferation and gene expression associated with cell proliferation and apoptosis in MDA-MB-231 human breast cancer cells. The MDA-MB-231 cells were cultured in the presence or absence of various concentrations (100, 200, or 300 µg/mL) of ERL. ERL significantly decreased cell proliferation after 48 h of incubation (P < 0.05). The protein and mRNA expression of ErbB(2) were decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of ErbB(3) was decreased significantly at an ERL concentration of 300 µg/mL (P < 0.05), and mRNA expression of ErbB(3) was decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of Akt was decreased significantly at the ERL concentration of 200 µg/mL (P < 0.05), and the protein expression of pAkt was decreased significantly in a dose-dependent manner (P < 0.05). The mRNA expression of Akt was decreased significantly at the ERL concentration of 200 µg/mL ERL (P < 0.05). The protein and mRNA expression of Bax were increased significantly at ERL concentrations of 200 µg/mL or higher (P < 0.05). The protein expression of Bcl(2) was increased significantly at ERL concentrations of 100 µg/mL or higher (P < 0.05), and mRNA expression of Bcl(2) was increased significantly at an ERL concentration of 300 µg/mL (P < 0.05). In conclusion, we suggest that Raphanus sativus, L. inhibits cell proliferation via the ErbB-Akt pathway in MDA-MB-231 cells.

摘要

在这项研究中，我们研究了萝卜地上部分乙醇提取物（ERL）对人乳腺癌 MDA-MB-231 细胞增殖以及与细胞增殖和凋亡相关基因表达的影响。将 MDA-MB-231 细胞在含有不同浓度（100、200 或 300µg/ml）ERL 的条件下培养。结果发现，与对照组相比，ERL 孵育 48 小时后显著降低细胞增殖（P<0.05）。ERB2 蛋白和 mRNA 表达呈剂量依赖性显著降低（P<0.05）。300µg/ml ERL 时 ERB3 蛋白表达显著降低（P<0.05），并且 ERB3 mRNA 表达呈剂量依赖性显著降低（P<0.05）。200µg/ml ERL 时 Akt 蛋白表达显著降低（P<0.05），并且 pAkt 蛋白表达呈剂量依赖性显著降低（P<0.05）。200µg/ml ERL 时 Akt mRNA 表达显著降低（P<0.05）。200µg/ml 或更高浓度的 ERL 时 Bax 蛋白和 mRNA 表达显著增加（P<0.05）。100µg/ml 或更高浓度的 ERL 时 Bcl2 蛋白表达显著增加（P<0.05），并且 300µg/ml ERL 时 Bcl2 mRNA 表达显著增加（P<0.05）。总之，我们认为萝卜通过 ErbB-Akt 通路抑制 MDA-MB-231 细胞的增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/d344e80ffb51/nrp-5-288-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/1334660b112a/nrp-5-288-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/53f18a868e77/nrp-5-288-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/b83c88dafb01/nrp-5-288-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/cfe754ad598b/nrp-5-288-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/d344e80ffb51/nrp-5-288-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/1334660b112a/nrp-5-288-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/53f18a868e77/nrp-5-288-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/b83c88dafb01/nrp-5-288-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/6504a7207aa5/nrp-5-288-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/cfe754ad598b/nrp-5-288-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ac/3180678/d344e80ffb51/nrp-5-288-g006.jpg

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