Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Chem Soc Rev. 2013 Jul 21;42(14):6032-59. doi: 10.1039/c3cs60055f.
Because of the biological importance of thiols, the development of probes for thiols has been an active research area in recent years. In this review, we summarize the results of recent exciting reports regarding thiol-addition reactions and their applications in thiol recognition. The examples reported can be classified into four reaction types including 1,1, 1,2, 1,3, 1,4 addition reactions, according to their addition mechanisms, based on different Michael acceptors. In all cases, the reactions are coupled to color and/or emission changes, although some examples dealing with electrochemical recognition have also been included. The use of thiol-addition reactions is a very simple and straightforward procedure for the preparation of thiol-sensing probes.
由于巯基在生物学上的重要性，近年来，开发用于检测巯基的探针一直是一个活跃的研究领域。在这篇综述中，我们总结了近年来关于巯基加成反应及其在巯基识别中的应用的令人兴奋的报告结果。根据不同的迈克尔受体，根据加成机制，报道的例子可以分为 1,1,1,2,1,3,1,4 加成反应四种类型。在所有情况下，反应都伴随着颜色和/或发射变化，尽管也包括了一些涉及电化学识别的例子。巯基加成反应是制备巯基传感探针的一种非常简单和直接的方法。
