Department of Chemistry, Korea University, Seoul 130-701, Korea.
Chem Soc Rev. 2013 Jul 21;42(14):6019-31. doi: 10.1039/c3cs60024f.
In the past few decades, the development of optical probes for thiols has attracted great attention because of the biological importance of the thiol-containing molecules such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH). This tutorial review focuses on various thiol detection methods based on luminescent or colorimetric spectrophotometry published during the period 2010-2012. The discussion covers a diversity of sensing mechanisms such as Michael addition, cyclization with aldehydes, conjugate addition-cyclization, cleavage of sulfonamide and sulfonate esters, thiol-halogen nucleophilic substitution, disulfide exchange, native chemical ligation (NCL), metal complex-displace coordination, and nanomaterial-related and DNA-based chemosensors.
在过去的几十年中，由于含巯基分子如半胱氨酸 (Cys)、高半胱氨酸 (Hcy) 和谷胱甘肽 (GSH) 的生物重要性，用于检测硫醇的光学探针的发展引起了极大的关注。本综述重点介绍了 2010 年至 2012 年期间发表的基于荧光或比色分光光度法的各种硫醇检测方法。讨论涵盖了多种传感机制，如迈克尔加成、与醛的环化、共轭加成-环化、磺酰胺和磺酸盐酯的裂解、硫醇-卤亲核取代、二硫键交换、天然化学连接 (NCL)、金属配合物置换配位以及与纳米材料和 DNA 相关的化学传感器。
