Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestr,15, 21071 Hamburg, Germany.
AMB Express. 2013 May 24;3(1):29. doi: 10.1186/2191-0855-3-29.
: In this work a defined co-culture of the lactic acid bacterium Lactobacillus zeae and the propionate producer Veillonella criceti has been studied in continuous stirred tank reactor (CSTR) and in a dialysis membrane reactor. It is the first time that this reactor type is used for a defined co-culture fermentation. This reactor allows high mixing rates and working with high cell densities, making it ideal for co-culture investigations. In CSTR experiments the co-culture showed over a broad concentration range an almost linear correlation in consumption and production rates to the supply with complex nutrients. In CSTR and dialysis cultures a strong growth stimulation of L. zeae by V. criceti was shown. In dialysis cultures very high propionate production rates (0.61 g L-1h-1) with final titers up to 28 g L-1 have been realized. This reactor allows an individual, intracellular investigation of the co-culture partners by omic-technologies to provide a better understanding of microbial communities.
在这项工作中，已在连续搅拌釜式反应器（CSTR）和透析膜式反应器中研究了产乳酸细菌植物乳杆菌和丙酸产生菌戊糖片球菌的定义共培养物。这是第一次将这种反应器类型用于定义共培养发酵。该反应器允许高混合速率和高细胞密度工作，使其成为共培养研究的理想选择。在 CSTR 实验中，共培养物在较宽的浓度范围内，消耗和生产速率与复杂营养素的供应几乎呈线性相关。在 CSTR 和透析培养中，戊糖片球菌对植物乳杆菌的生长有很强的刺激作用。在透析培养中，实现了非常高的丙酸生产速率（0.61 g L-1h-1），最终浓度高达 28 g L-1。该反应器允许通过组学技术对共培养物伙伴进行单独的细胞内研究，以更好地了解微生物群落。
