Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, California 90089, USA.
Org Lett. 2013 Jun 7;15(11):2862-5. doi: 10.1021/ol401187b. Epub 2013 May 24.
Aspernidine A is a prenylated isoindolinone alkaloid isolated from the model fungus Aspergillus nidulans. A genome-wide kinase knockout library of A. nidulans was examined, and it was found that a mitogen-activated protein kinase gene, mpkA, deletion strain produces aspernidine A. Targeted gene deletions were performed in the kinase deletion background to identify the gene cluster for aspernidine A biosynthesis. Intermediates were isolated from mutant strains which provided information about the aspernidine A biosynthesis pathway.
阿朴啡 A 是一种从模式真菌构巢曲霉中分离得到的异吲哚啉酮生物碱。对构巢曲霉的全基因组激酶敲除文库进行了检查，发现一个丝裂原活化蛋白激酶基因 mpkA 缺失菌株会产生阿朴啡 A。在激酶缺失背景下进行了靶向基因缺失，以鉴定阿朴啡 A 生物合成的基因簇。从突变株中分离出了中间体，这些中间体提供了关于阿朴啡 A 生物合成途径的信息。
