人类乙肝病毒基因组adr血清型的克隆与限制性酶切图谱分析
Cloning and restriction mapping of human HBV genome serotype adr.
作者信息
Wu X F, Zhou Y Z, Feng Z M, Li Z P, Xia S Y
出版信息
Sci Sin B. 1983 Sep;26(9):954-60.
A Bam HI cleaved 3.2 Kb fragment from the HBV adr genome was cloned in E. coli using pBR322 as vector. Sixteen restriction sites from the action of Bam HI, Hind III, Bgl I, Bgl II, Ava I, Hinc II, Sph I, Xba I, Xho I and Sst II were determined and mapped. No cleavage sites were found for Eco RI, Pst I, Sma I, Hpa I, Kpn I, Pvu II and Sst I. The restriction map for HBV adr is significantly different from those reported for the subtypes adw, ayw and adyw.
使用pBR322作为载体，将来自乙肝病毒adr基因组的一个经Bam HI酶切的3.2 Kb片段克隆到大肠杆菌中。确定并绘制了Bam HI、Hind III、Bgl I、Bgl II、Ava I、Hinc II、Sph I、Xba I、Xho I和Sst II作用产生的16个限制性酶切位点。未发现Eco RI、Pst I、Sma I、Hpa I、Kpn I、Pvu II和Sst I的酶切位点。乙肝病毒adr的限制性酶切图谱与报道的adw、ayw和adyw亚型的图谱有显著差异。
Zotero 插件