Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
Microb Pathog. 2013 Aug-Sep;61-62:57-61. doi: 10.1016/j.micpath.2013.05.006. Epub 2013 May 21.
ATP-binding cassette transporter A1 (ABCA1) mediates reverse cholesterol transport and innate immunity response in different cell types. We have investigated the regulation of ABCA1 expression in response to intracellular Chlamydia pneumoniae infection in A549 epithelial lung carcinoma cells. C. pneumoniae infection decreased ABCA1 expression in A549 cells, and the activity of the ABCA1 promoter was decreased. The decreased promoter activity was dependent on its E-box and GnT-box elements of the promoter. Chlamydial growth was decreased in ABCA1-silenced epithelial lung carcinoma cells. These data indicate an important role for ABCA1 in intracellular bacterial infection.
三磷酸腺苷结合盒转运体 A1（ABCA1）在不同细胞类型中介导胆固醇逆向转运和先天免疫反应。我们研究了细胞内肺炎衣原体感染对 A549 上皮肺癌细胞中 ABCA1 表达的调节。肺炎衣原体感染降低了 A549 细胞中的 ABCA1 表达，并且 ABCA1 启动子的活性降低。降低的启动子活性依赖于其启动子的 E 盒和 GnT 盒元件。在沉默 ABCA1 的上皮肺癌细胞中，衣原体的生长减少。这些数据表明 ABCA1 在细胞内细菌感染中起重要作用。
