Department of Chemistry, Wayne State University, 5101 Cass Ave., Detroit, MI, United States.
Methods. 2013 Sep 15;63(2):144-59. doi: 10.1016/j.ymeth.2013.04.023. Epub 2013 May 23.
To regulate stress responses and virulence, bacteria use small regulatory RNAs (sRNAs). These RNAs can up or down regulate target mRNAs through base pairing by influencing ribosomal access and RNA decay. A large class of these sRNAs, called trans-encoded sRNAs, requires the RNA binding protein Hfq to facilitate base pairing between the regulatory RNA and its target mRNA. The resulting network of regulation is best characterized in Escherichia coli and Salmonella typhimurium, but the importance of Hfq dependent sRNA regulation is recognized in a diverse population of bacteria. In this review we present the approaches and methods used to discover Hfq binding RNAs, characterize their interactions and elucidate their functions.
为了调节应激反应和毒力，细菌使用小的调节 RNA（sRNA）。这些 RNA 可以通过影响核糖体进入和 RNA 降解来通过碱基配对上调或下调靶 mRNA。这些 sRNA 的一大类，称为转编码 sRNA，需要 RNA 结合蛋白 Hfq 来促进调节 RNA 与其靶 mRNA 之间的碱基配对。这种调节的网络在大肠杆菌和鼠伤寒沙门氏菌中得到了很好的描述，但在不同种类的细菌中也认识到了 Hfq 依赖性 sRNA 调节的重要性。在这篇综述中，我们介绍了发现 Hfq 结合 RNA 的方法和方法，描述了它们的相互作用，并阐明了它们的功能。
