Hyeon Jong Woo, Kim Kyung Hyun, Jeong Sang Eun, Jeon Che Ok
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3627-3632. doi: 10.1099/ijsem.0.002181. Epub 2017 Sep 6.
A Gram-stain-negative, strictly aerobic bacterium, designated KJ21T, was isolated from a tidal flat in South Korea. Cells were non-motile rods showing oxidase- and catalase-positive activities. Growth of strain KJ21T was observed at 10-35 °C (optimum, 30 °C), at pH 6.0-8.5 (optimum, pH 7.0) and in the presence of 1-5 % (w/v) NaCl (optimum, 2 %). Strain KJ21T contained summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c), C16 : 0, 10-methyl C19 : 0 and C10 : 0 3-OH as the major fatty acids and ubiquinone-10 as the major isoprenoid quinone. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unknown aminolipid and an unknown lipid were detected as the major polar lipids. The G+C content of the genomic DNA was 53.9 mol%. Phylogenic analysis based on 16S rRNA gene sequences showed that strain KJ21T formed a tight phylogenetic lineage with the members of the genus Pacificibacter with a 100 % bootstrap value. Strain KJ21T was most closely related to Pacificibacter maritimus KMM 9031T (98.7 %) and Pacificibacter marinus HDW-9T (98.4 %), and the DNA-DNA relatedness values between strain KJ21T and the type strains of P. maritimus and P. marinus were 46.9±4.2 % and 39.8±5.7, respectively. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain KJ21T represents a novel species of the genus Pacificibacter, for which the name Pacificibacter aestuarii sp. nov. is proposed. The type strain is KJ21T (=KACC 19098T=JCM 31805T).
从韩国一处潮滩分离出一株革兰氏染色阴性、严格需氧的细菌，命名为KJ21T。细胞为无运动性的杆菌，氧化酶和过氧化氢酶活性呈阳性。观察到菌株KJ21T在10 - 35 °C（最适温度30 °C）、pH 6.0 - 8.5（最适pH 7.0）以及1 - 5 %（w/v）NaCl（最适浓度2 %）条件下生长。菌株KJ21T的主要脂肪酸包括特征性总和8（包含C18 : 1ω7c/C18 : 1ω6c）、C16 : 0、10 - 甲基C19 : 0和C10 : 0 3 - OH，主要类异戊二烯醌为泛醌 - 10。检测到磷脂酰甘油、双磷脂酰甘油、磷脂酰胆碱、一种未知氨基脂质和一种未知脂质为主要极性脂质。基因组DNA的G + C含量为53.9 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株KJ21T与太平洋杆菌属成员形成了一个紧密的系统发育分支，自展值为100 %。菌株KJ21T与海洋太平洋杆菌KMM 9031T（98.7 %）和海生太平洋杆菌HDW - 9T（98.4 %）关系最为密切，菌株KJ21T与海洋太平洋杆菌和海生太平洋杆菌模式菌株之间的DNA - DNA相关性值分别为46.9±4.2 %和39.8±5.7。基于表型、化学分类和分子特性，很明显菌株KJ21T代表太平洋杆菌属的一个新物种，为此提出新名称河口太平洋杆菌。模式菌株为KJ21T（=KACC 19098T=JCM 31805T）。
